Pevzner L Z, Iakoubek B
Tsitologiia. 1978 Feb;20(2):225-8.
Anticipation stress was induced in 16 day-old male rats by placing the animals daily for 7 days into individual cells for 45 min. In the end of each 45 min session, an electric stimulation of paws of the animals was done for 2 min. It was shown by visible cytospectrophotometry of amido black-stained spinal cord sections that the anticipation stress for 7 days resulted in an accumulation of the nuclear and cytoplasmic total proteins in the motoneurons of spinal cord anterior horns, with no changes in the body (in fact, in the nuclei) of the glial cells adjacent to the neurons. Intraperitoneal injection of the tranquilizer diazepam (10 mg per kg) 40 min. before the beginning of the last anticipation stress session gave rise to the return to the normal of the protein content per cell in the motoneuron nucleus and cytoplasm while inducing an increase in the quantity of neuroglia cell protein. Differences in the protein metabolism between the neurons and the neuroglia are discussed.
通过将16日龄雄性大鼠每天置于单独的笼子中45分钟，持续7天，诱导其产生预期应激。在每次45分钟的实验结束时，对动物的爪子进行2分钟的电刺激。通过对氨基黑染色的脊髓切片进行可见细胞分光光度法显示，7天的预期应激导致脊髓前角运动神经元的细胞核和细胞质总蛋白积累，而与神经元相邻的神经胶质细胞的主体（实际上是细胞核）没有变化。在最后一次预期应激实验开始前40分钟腹腔注射安定（每公斤10毫克），可使运动神经元细胞核和细胞质中每个细胞的蛋白质含量恢复正常，同时诱导神经胶质细胞蛋白质数量增加。文中讨论了神经元和神经胶质细胞之间蛋白质代谢的差异。
