Hirshfield A N, De Paolo L V
J Endocrinol. 1981 Jan;88(1):67-71. doi: 10.1677/joe.0.0880067.
To examine the manner in which the FSH surge of one oestrous cycle recruits follicles for ovulation in the subsequent cycle, porcine follicular fluid (PFF) was used to alter the pattern of endogenous FSH secretion during the periovulatory period. OVaries of animals killed at oestrus or metoestrus were examined histologically for the presence of large follicles (greater than 400 micrometers in diameter) after treatment. Large follicles were absent in ovaries of PFF-treated animals at oestrus, while control rats had an average of 2.7 large follicles per ovary. By metoestrus, however, ovaries of rats treated with PFF contained several large, healthy follicles. Only when PFF treatment was continued throughout the evening of oestrus was the appearance of large follicles prevented at metoestrus. Our results suggest that the prolonged oestrus portion of the FSH surge, rather than the pro-oestrous portion, is responsible for follicular recruitment during the normal oestrous cycle in the rat. They also indicate that compensatory follicular development occurs in response to the FSH rebound which has been shown to follow FSH suppression.
为了研究一个发情周期中的促卵泡素（FSH）高峰促使卵泡在随后的周期中排卵的方式，使用猪卵泡液（PFF）来改变排卵前期内源性FSH的分泌模式。对在发情期或发情后期处死的动物的卵巢进行组织学检查，以观察处理后大卵泡（直径大于400微米）的存在情况。在发情期，接受PFF处理的动物卵巢中没有大卵泡，而对照大鼠每个卵巢平均有2.7个大卵泡。然而，到发情后期，接受PFF处理的大鼠卵巢中含有几个大的、健康的卵泡。只有当在整个发情期晚上持续进行PFF处理时，发情后期才会阻止大卵泡的出现。我们的结果表明，FSH高峰中延长的发情期部分而非发情前期部分，是大鼠正常发情周期中卵泡募集的原因。它们还表明，补偿性卵泡发育是对已证明在FSH抑制后出现的FSH反弹的反应。
