Ahmed M, Schidlovsky G
J Virol. 1968 Dec;2(12):1443-57. doi: 10.1128/JVI.2.12.1443-1457.1968.
Upon cultivation of chicken lymphoid tumors induced by Georgia isolate of Marek's disease, clusters of refractile rounded cells appeared consistently in the cell cultures. Intranuclear mature, immature, enveloped, and empty herpesvirus-type particles, usually associated with smaller particles, were observed in all rounded cells in the affected areas. Cytoplasmic inclusions containing mature enveloped virus particles were also seen in the rounded cells. Often fibroblasts growing in the vicinity of the cytopathic effect area contained a few herpesvirus-type particles. The original tumors prior to cultivation did not reveal any virus particles, and the virus in infected cultures was always cell-associated. Control cell cultures neither developed the rounded cells nor demonstrated the presence of any type of virus particles.
用马立克氏病佐治亚分离株诱导鸡产生淋巴肿瘤后，在细胞培养物中始终会出现成簇的折光性圆形细胞。在受影响区域的所有圆形细胞中均观察到核内成熟、不成熟、有包膜和无包膜的疱疹病毒样颗粒，这些颗粒通常与较小的颗粒相关。在圆形细胞中还可见到含有成熟有包膜病毒颗粒的细胞质包涵体。在细胞病变效应区域附近生长的成纤维细胞通常含有少量疱疹病毒样颗粒。培养前的原始肿瘤未显示任何病毒颗粒，感染培养物中的病毒始终与细胞相关。对照细胞培养物既未形成圆形细胞，也未显示任何类型病毒颗粒的存在。
