Roos E, Dingemans K P, van de Pavert I V, van den Bergh-Weerman M
J Natl Cancer Inst. 1977 Feb;58(2):399-407. doi: 10.1093/jnci/58.2.399.
Liver of (C57BL X DBA)F1 mice were perfused in situ with a synthetic hemoglobin-free medium, to which murine lymphosarcoma cells were added. All cells were arrested. They were only found in the sinusoids, predominantly in periportal areas. Many lymphosarcoma cells penetrated the walls of the sinusoids with protrusions that extended into and through the endothelial cells. The protrusions often also invaded hepatocytes, and some cells migrated out of the sinusoids. The percentage of the cells that penetrated endothelium was constant and reproducible (68 +/- 4%) in experiments lasting longer than 90 minutes, but the percentage of these cells that also invaded hepatocytes varied greatly. Parellel experiments in vivo yielded similar results, except that the number of cells that invaded hepatocytes was generally much lower. The advantages of the perfused liver as a model for experimental study of the invasion mechanism were evaluated.
用无合成血红蛋白的培养基对（C57BL×DBA）F1小鼠的肝脏进行原位灌注，并向其中加入鼠淋巴肉瘤细胞。所有细胞均停滞。它们仅存在于肝血窦中，主要在门静脉周围区域。许多淋巴肉瘤细胞通过延伸进入并穿过内皮细胞的突起穿透肝血窦壁。这些突起常常还侵入肝细胞，并且一些细胞从肝血窦中迁移出来。在持续超过90分钟的实验中，穿透内皮细胞的细胞百分比是恒定且可重复的（68±4%），但这些也侵入肝细胞的细胞百分比差异很大。体内的平行实验得出了类似的结果，只是侵入肝细胞的细胞数量通常要低得多。评估了灌注肝脏作为侵袭机制实验研究模型的优点。
