Bronson D L, Bookout J, Bergs V V, Haines H G
Proc Soc Exp Biol Med. 1976 May;152(1):116-21. doi: 10.3181/00379727-152-39340.
The non-oncogenic rat C-type virus WF-1, isolated from a Wistar-Furth rat embryo cell line, was characterized biochemically. The purified virus has a buoyant density of 1.15 to 1.16 g/cm3 in sucrose, RNA-dependent DNA polymerase (reverse transcriptase) activity, and RNA with a sedimentation coefficient of 62 to 68 S. The viral RNA is single-stranded, and, upon treatment with heat, yields components with sedimentation coefficients of 36 S, 18 to 20 S, and 4 to 12 S.
从Wistar-Furth大鼠胚胎细胞系中分离出的非致癌性大鼠C型病毒WF-1进行了生化特性分析。纯化后的病毒在蔗糖中的浮力密度为1.15至1.16 g/cm3,具有RNA依赖性DNA聚合酶(逆转录酶)活性,以及沉降系数为62至68 S的RNA。病毒RNA为单链,经加热处理后产生沉降系数为36 S、18至20 S和4至12 S的组分。