Bjorklid E, Storm-Mathisen J, Storm E, Prydz H
Thromb Haemost. 1977 Feb 28;37(1):91-7.
Monospecific antisera against the purified protein component of tissue thromboplastin (apoprotein-III) from human brain have been raised in goats and rabbits. The antisera neutralized tissue thromboplastin prepared from brain, thyroid gland and pulmonary tissue, indicating that apoproteins in the various preparations cross-reacted immunologically and therefore were similar or identical. Comparison of the activities of tissue thromboplastin preparations from 34 different areas of the brain demonstrated a characteristic distribution pattern and a wide range of activities. White and grey matter from the same areas had similar activities. Bulbus and tractus olfactorius, medulla oblongata, corpus pineale, hippocampus and hypothalamus contained 160-270% of the average activity, whereas cerebellum globus pallidus, nucleus ruber and substantia nigra contained 30-60%. The distinct distribution pattern was unrelated to tissue vascularization, and may suggest that apoprotein-III could serve other functions, apart from the coagulation of blood. The predominance in phylogenetically older brain regions would suggest that it represents a primitive or fundamental feature.
已在山羊和兔子体内制备出针对人脑组织凝血活酶（脱辅基蛋白III）纯化蛋白成分的单特异性抗血清。这些抗血清可中和从脑、甲状腺和肺组织制备的组织凝血活酶，这表明不同制剂中的脱辅基蛋白存在免疫交叉反应，因此它们相似或相同。对来自大脑34个不同区域的组织凝血活酶制剂活性进行比较，结果显示出一种特征性的分布模式以及广泛的活性范围。同一区域的白质和灰质具有相似的活性。嗅球和嗅束、延髓、松果体、海马体和下丘脑的活性为平均活性的160 - 270%，而小脑、苍白球、红核和黑质的活性为30 - 60%。这种明显的分布模式与组织血管化无关，这可能表明脱辅基蛋白III除了参与血液凝固外，还可能具有其他功能。在系统发育较古老的脑区中占主导地位，这表明它代表了一种原始或基本特征。
