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电子显微镜在贾第虫病诊断中的应用

Electron microscopy in the diagnosis of giardiasis.

作者信息

Klima M, Gyorkey P, Min K W, Gyorkey F

出版信息

Arch Pathol Lab Med. 1977 Mar;101(3):133-5.

PMID:576576
Abstract

Biopsy specimens of intestinal mucosa from two patients with malabsorption syndrome were examined with routine light and electron microscopy. Both showed intact intestinal mucosa, but by electron microscopy, the presence of Giardia lamblia in the intervillar spaces was identified. Electron microscopy is useful for the identification of this parasite, since the stool examinations in up to 50% may be negative. The parasites appear in light microscopy as cellular debris and can be easily overlooked, while by electron microscopy the typical morphologic features of the parasite are diagnostic.

摘要

对两名吸收不良综合征患者的肠黏膜活检标本进行了常规光学显微镜和电子显微镜检查。两人的肠黏膜均完整,但电子显微镜检查发现绒毛间隙存在蓝氏贾第鞭毛虫。电子显微镜有助于识别这种寄生虫,因为高达50%的粪便检查可能为阴性。在光学显微镜下,这些寄生虫表现为细胞碎片,很容易被忽视,而通过电子显微镜,寄生虫典型的形态特征具有诊断价值。

相似文献

1
Electron microscopy in the diagnosis of giardiasis.电子显微镜在贾第虫病诊断中的应用
Arch Pathol Lab Med. 1977 Mar;101(3):133-5.
2
[The value of the "impression smear" in detecting giardia lamblia infection (author's transl)].“压片涂片”在检测蓝氏贾第鞭毛虫感染中的价值(作者译)
Padiatr Padol. 1978;13(2):165-73.
3
[Malabsorption syndrome caused by Giardia lamblia in gastrectomized patients].胃切除术后患者由蓝氏贾第鞭毛虫引起的吸收不良综合征
Enferm Infecc Microbiol Clin. 1989 Feb;7(2):100-2.
4
A comparative study of four methods for detecting Giardia lamblia in children with diarrheal disease and malabsorption.四种检测腹泻病和吸收不良患儿中蓝氏贾第鞭毛虫方法的比较研究。
Gastroenterology. 1974 Jan;66(1):16-21.
5
Coinfection with Giardia lamblia and Enterocytozoon bieneusi in a patient with acquired immunodeficiency syndrome and chronic diarrhea.一名获得性免疫缺陷综合征和慢性腹泻患者同时感染蓝氏贾第鞭毛虫和微小隐孢子虫。
Arch Pathol Lab Med. 1997 Apr;121(4):417-22.
6
C-kit (CD117) immunostain is useful for the diagnosis of Giardia lamblia in duodenal biopsies.C-kit(CD117)免疫染色有助于十二指肠活检中贾第虫的诊断。
Hum Pathol. 2009 Mar;40(3):323-5. doi: 10.1016/j.humpath.2008.07.015. Epub 2008 Oct 5.
7
[Giardiasis in children. Ultrastructural study of the parasite].
Pediatr Med Chir. 1983 Jul-Aug;5(4):189-93.
8
Diagnostic value of endoscopy for the diagnosis of giardiasis and other intestinal diseases in patients with persistent diarrhea from tropical or subtropical areas.内镜检查对热带或亚热带地区持续性腹泻患者贾第虫病及其他肠道疾病的诊断价值。
Scand J Gastroenterol. 2007 Mar;42(3):391-6. doi: 10.1080/00365520600881193.
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[Diagnostic limitations of the isolation of trophozoites of Giardia lamblia in duodenal aspirates].[十二指肠抽吸物中分离蓝氏贾第鞭毛虫滋养体的诊断局限性]
Arq Gastroenterol. 1994 Apr-Jun;31(2):69-74.
10
[A proposal for the classification of intestinal complications in giardiasis].
Acta Gastroenterol Latinoam. 1986;16(1):27-31.

引用本文的文献

1
From Leningrad to the day-care center. The ubiquitous Giardia lamblia.从列宁格勒到日托中心。无处不在的蓝氏贾第鞭毛虫。
West J Med. 1990 Aug;153(2):154-9.
2
Prevalence of Giardia spp. in beaver and muskrat populations in northeastern states and Minnesota: detection of intestinal trophozoites at necropsy provides greater sensitivity than detection of cysts in fecal samples.美国东北部各州和明尼苏达州海狸和麝鼠种群中贾第虫属的流行情况:尸检时检测肠道滋养体比检测粪便样本中的包囊具有更高的灵敏度。
Appl Environ Microbiol. 1990 Jan;56(1):31-6. doi: 10.1128/aem.56.1.31-36.1990.