Jelínek J, Jelínková M
Acta Endocrinol (Copenh). 1977 May;85(1):169-76.
The specific activities of lactate dehydrogenase (LDH) and its M-type (M-LDH), beta-glucuronidase (beta-GR), acid phosphatase (ACP) and alkaline phosphatase (AP) were determined. The specific activities of the enzymes (LDH, beta-GR) in the myometrium were lower and their changes less pronounced than in the endometrium. We, therefore, determined the enzymes in the rat endometrium only in further experiments. All enzymes react sensitively to the changes induced in the endometrium by endogenous hormones in the course of a 4-day cycle: pro-oestrus (P) is characterized by rather low enzyme activities, oestrus (E) by a peak of LDH and M-LDH and a rise of AP. In metoestrus (M) there is a peak of beta-GR, ACP and AP. Dioestrus (D) is characterized by a significant decrease in LDH and M-LDH and by elevated values of all the other enzymes. The values on the individual days of the 4-day cycle were compared with days 4-6 of pregnancy. The reason for this was that if the rats were not mated, they would, respectively, return to pro-oestrus instead of being 4 days pregnant, to oestrus instead of being 5 days pregnant, or to metoestrus instead of being 6 days pregnant. We found the following differences: on day 4 of pregnancy LDH and M-LDH were lower and ACP and AP higher than in P. On day 5 of pregnancy the LDH, M-LDH, beta-GR and AP were lower than in E. On day 6 of pregnancy the LDH, M-LDH, ACP and especially beta-GR, were lower than in M.
测定了乳酸脱氢酶（LDH）及其M型（M-LDH）、β-葡萄糖醛酸酶（β-GR）、酸性磷酸酶（ACP）和碱性磷酸酶（AP）的比活性。子宫肌层中这些酶（LDH、β-GR）的比活性低于子宫内膜，且其变化不如子宫内膜明显。因此，在后续实验中我们仅测定了大鼠子宫内膜中的这些酶。所有酶对4天周期内子宫内膜由内源性激素诱导的变化反应敏感：动情前期（P）的特点是酶活性相当低，发情期（E）的特点是LDH和M-LDH达到峰值且AP升高。间情期（M）时β-GR、ACP和AP出现峰值。动情后期（D）的特点是LDH和M-LDH显著降低，而其他所有酶的值升高。将4天周期中各天的值与妊娠第4 - 6天进行了比较。这样做的原因是，如果大鼠未交配，它们会分别回到动情前期而非妊娠4天、回到发情期而非妊娠5天或回到间情期而非妊娠6天。我们发现了以下差异：妊娠第4天，LDH和M-LDH低于动情前期，而ACP和AP高于动情前期。妊娠第5天，LDH、M-LDH、β-GR和AP低于发情期。妊娠第6天，LDH、M-LDH、ACP尤其是β-GR低于间情期。
