Jelinek J, Ylikorkala O, Jelínková M, Järvinen P A, Alapiessa U
Int J Fertil. 1978;23(1):31-7.
Activities of 9 enzymes were determined biochemically in the endometrium. In Trial I (five women) 25 mg progesterone were injected i.m. on day 9 of the cycle; and endometrial biopsy taken 24 hours later was compared with endometrium from day 10 and day 21, taken in two untreated cycles from the same volunteers. Similarly, in Trial II (five women) 50 mg progesterone were injected on day 9, biopsy taken on day 11 and compared with days 11 and 21 from untreated cycles. The specific activites of lactate dehydrogenase, isocitrate dehydrogenase (ICDH), malate dehydrogenase, glutamate dehydrogenase, beta-glucuronidase, acid phosphatase (ACP) and alkaline phosphatase (AP) were significantly higher in the secretory phase. Twenty-five milligrams progesterone (after 24 hours) caused increases of some enzymes, significant only for AP. Fifty milligrams (after 48 hours) increased significantly the activity of ICDH and ACP. Biochemical changes, especially increase of ICDH, can be used for detection of the effect of progesterone on the endometrium.
对子宫内膜中的9种酶的活性进行了生化测定。在试验I（5名女性）中，在月经周期的第9天肌肉注射25毫克孕酮；24小时后进行子宫内膜活检，并与同一志愿者在两个未治疗周期的第10天和第21天获取的子宫内膜进行比较。同样，在试验II（5名女性）中，在第9天注射50毫克孕酮，在第11天进行活检，并与未治疗周期的第11天和第21天进行比较。乳酸脱氢酶、异柠檬酸脱氢酶（ICDH）、苹果酸脱氢酶、谷氨酸脱氢酶、β-葡萄糖醛酸酶、酸性磷酸酶（ACP）和碱性磷酸酶（AP）的比活性在分泌期显著更高。25毫克孕酮（24小时后）使某些酶增加，仅AP的增加具有显著性。50毫克（48小时后）使ICDH和ACP的活性显著增加。生化变化，尤其是ICDH的增加，可用于检测孕酮对子宫内膜的作用。
