表现出淀粉酶和转化酶超量产生的粗糙脉孢菌突变体。
Neurospora mutant exhibiting hyperproduction of amylase and invertase.
作者信息
Gratzner H, Sheehan D N
出版信息
J Bacteriol. 1969 Feb;97(2):544-9. doi: 10.1128/jb.97.2.544-549.1969.
Abstract
A mutant strain of Neurospora crassa has been isolated which is derepressed for amylase and beta-fructofuranosidase (invertase). Large amounts of the two enzymes were secreted into the culture medium upon depletion of exogenous carbon source. The resulting increases of the two extracellular enzymes were prevented by actinomycin D, cycloheximide, and glycerol. The starving cells of the mutant strain produced amylase and invertase de novo, as evidenced by incorporation of radioactive amino acids into the enzymes. Preliminary genetic studies indicate that these elevated enzyme levels described are due to a single gene mutation.
摘要
已分离出一种粗糙脉孢菌的突变菌株，该菌株的淀粉酶和β-呋喃果糖苷酶（转化酶）处于去阻遏状态。在外源碳源耗尽时，大量的这两种酶被分泌到培养基中。放线菌素D、环己酰亚胺和甘油可阻止这两种细胞外酶的增加。突变菌株的饥饿细胞从头合成淀粉酶和转化酶，这可通过放射性氨基酸掺入这些酶中得到证明。初步的遗传学研究表明，所描述的这些酶水平升高是由单一基因突变引起的。
相似文献
1
Neurospora mutant exhibiting hyperproduction of amylase and invertase.
J Bacteriol. 1969 Feb;97(2):544-9. doi: 10.1128/jb.97.2.544-549.1969.
2
Cell wall alterations associated with the hyperproduction of extracellular enzymes in Neurospora crassa.
J Bacteriol. 1972 Aug;111(2):443-6. doi: 10.1128/jb.111.2.443-446.1972.
3
Hyperproduction of some glycosidases in Neurospora crassa.
Arch Microbiol. 1974;101(4):303-20. doi: 10.1007/BF00455947.
4
Molecular sieving by Neurospora cell walls during secretion of invertase isozymes.
J Bacteriol. 1966 Oct;92(4):1010-5. doi: 10.1128/jb.92.4.1010-1015.1966.
5
Regeneration of invertase in Neurospora crassa.
J Bacteriol. 1973 Jul;115(1):146-52. doi: 10.1128/jb.115.1.146-152.1973.
6
Isolation, mapping, and characterization of trehalaseless mutants of Neurospora crassa.
J Bacteriol. 1971 Oct;108(1):59-68. doi: 10.1128/jb.108.1.59-68.1971.
7
Control of trehalase synthesis in Neurospora crassa.
Am J Bot. 1969 Nov-Dec;56(10):1160-6.
8
Gene-enzyme relationships in Neurospora invertase.
J Bacteriol. 1969 Feb;97(2):867-72. doi: 10.1128/jb.97.2.867-872.1969.
9
Derepression of tyrosinase synthesis in Neurospora by cycloheximide, actinomycin D, and puromycin.
J Biol Chem. 1970 Jun 10;245(11):2784-8.
引用本文的文献
1
The F-box protein gene - is a target for reverse engineering enzyme hypersecretion in filamentous fungi.
Proc Natl Acad Sci U S A. 2021 Jun 29;118(26). doi: 10.1073/pnas.2025689118.
2
Establishment of Neurospora crassa as a host for heterologous protein production using a human antibody fragment as a model product.
Microb Cell Fact. 2017 Jul 25;16(1):128. doi: 10.1186/s12934-017-0734-5.
3
Regulation of cellulase and hemicellulase gene expression in fungi.
Curr Genomics. 2013 Jun;14(4):230-49. doi: 10.2174/1389202911314040002.
4
Semiquantitative Plate Assay for Determination of Cellulase Production by Trichoderma viride.
Appl Environ Microbiol. 1977 Jan;33(1):178-83. doi: 10.1128/aem.33.1.178-183.1977.
5
Lessons from the genome sequence of Neurospora crassa: tracing the path from genomic blueprint to multicellular organism.
Microbiol Mol Biol Rev. 2004 Mar;68(1):1-108. doi: 10.1128/MMBR.68.1.1-108.2004.
6
Chromosomal loci of Neurospora crassa.
Microbiol Rev. 1982 Dec;46(4):426-570. doi: 10.1128/mr.46.4.426-570.1982.
7
Modified substrates and modified products as inducers of carbohydrases.
J Bacteriol. 1969 Dec;100(3):1151-4. doi: 10.1128/jb.100.3.1151-1154.1969.
8
Temperature-dependent actinomycin D effect on RNA synthesis during synchronous development in Neurospora crassa.
Biochem Genet. 1971 Dec;5(6):521-32. doi: 10.1007/BF00485670.
9
Enhanced cellulase production by a mutant of Trichoderma viride.
Appl Microbiol. 1971 Jan;21(1):152-4. doi: 10.1128/am.21.1.152-154.1971.
10
Effect of maltose on glucoamylase formation by Aspergillus niger.
J Bacteriol. 1972 Sep;111(3):771-7. doi: 10.1128/jb.111.3.771-777.1972.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75.
2
A gene affecting the repression of invertase and trehalase in Neurospora.
Arch Biochem Biophys. 1962 Mar;96:468-74. doi: 10.1016/0003-9861(62)90322-3.
3
DEVELOPMENT OF TREHALASE AND INVERTASE ACTIVITY IN NEUROSPORA.
J Bacteriol. 1964 Dec;88(6):1556-66. doi: 10.1128/jb.88.6.1556-1566.1964.
4
The purification and properties of invertase of Neurospora.
Arch Biochem Biophys. 1963 Mar;100:503-11. doi: 10.1016/0003-9861(63)90118-8.
5
The glycogen-amylase complex as a means of obtaining highly purified alpha-amylases.
Biochim Biophys Acta. 1962 Dec 4;65:200-6. doi: 10.1016/0006-3002(62)91039-9.
6
Molecular sieving by Neurospora cell walls during secretion of invertase isozymes.
J Bacteriol. 1966 Oct;92(4):1010-5. doi: 10.1128/jb.92.4.1010-1015.1966.
7
The invertase isozyme formed by Neurospora protoplasts.
Biochem Biophys Res Commun. 1964 Jul 1;16(4):319-25. doi: 10.1016/0006-291x(64)90033-6.
Zotero 插件