表现出淀粉酶和转化酶超量产生的粗糙脉孢菌突变体。
Neurospora mutant exhibiting hyperproduction of amylase and invertase.
作者信息
Gratzner H, Sheehan D N
出版信息
J Bacteriol. 1969 Feb;97(2):544-9. doi: 10.1128/jb.97.2.544-549.1969.
Abstract
A mutant strain of Neurospora crassa has been isolated which is derepressed for amylase and beta-fructofuranosidase (invertase). Large amounts of the two enzymes were secreted into the culture medium upon depletion of exogenous carbon source. The resulting increases of the two extracellular enzymes were prevented by actinomycin D, cycloheximide, and glycerol. The starving cells of the mutant strain produced amylase and invertase de novo, as evidenced by incorporation of radioactive amino acids into the enzymes. Preliminary genetic studies indicate that these elevated enzyme levels described are due to a single gene mutation.
摘要
已分离出一种粗糙脉孢菌的突变菌株，该菌株的淀粉酶和β-呋喃果糖苷酶（转化酶）处于去阻遏状态。在外源碳源耗尽时，大量的这两种酶被分泌到培养基中。放线菌素D、环己酰亚胺和甘油可阻止这两种细胞外酶的增加。突变菌株的饥饿细胞从头合成淀粉酶和转化酶，这可通过放射性氨基酸掺入这些酶中得到证明。初步的遗传学研究表明，所描述的这些酶水平升高是由单一基因突变引起的。
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Molecular sieving by Neurospora cell walls during secretion of invertase isozymes.在转化酶同工酶分泌过程中，粗糙脉孢菌细胞壁的分子筛作用。
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