Trevithick J R, Metzenberg R L
J Bacteriol. 1966 Oct;92(4):1010-5. doi: 10.1128/jb.92.4.1010-1015.1966.
Trevithick, John R. (University of Wisconsin Medical School, Madison), and Robert L. Metzenberg. Molecular sieving by Neurospora cell walls during secretion of invertase isozymes. J. Bacteriol. 92: 1010-1015. 1966.-The secretion of invertase by young mycelia of Neurospora was studied. The process of secretion was found to be dependent upon growth. The results indicate that fractionation of light invertase, the monomer, from heavy invertase, the aggregated form, occurs at the cell wall. Neurospora strains wild type, crisp, osmotic, and the double mutant crisp osmotic were tested. An inverse relation exists between the fraction of the total invertase activity of the culture which the mold secretes into the medium and the degree of fractionation, defined as the ratio of the fraction of the invertase secreted into the medium that is light invertase to the fraction of the invertase remaining associated with the cells that is light invertase. The hypothesis is offered that the increased secretion of invertase and decreased degree of fractionation seen in osmotic mutants, and to a lesser extent in the other mutants, can be explained by an increased porosity of the cell wall.
特里维西克，约翰·R.（威斯康星大学医学院，麦迪逊），以及罗伯特·L.梅岑伯格。在转化酶同工酶分泌过程中粗糙脉孢菌细胞壁的分子筛作用。《细菌学杂志》92: 1010 - 1015。1966年。——对粗糙脉孢菌幼菌丝体分泌转化酶的过程进行了研究。发现分泌过程依赖于生长。结果表明，单体形式的轻转化酶与聚集形式的重转化酶在细胞壁处发生分离。对野生型、脆壁型、渗透型粗糙脉孢菌菌株以及双突变体脆壁渗透型进行了测试。霉菌分泌到培养基中的转化酶占培养物总转化酶活性的比例与分离程度之间存在反比关系，分离程度定义为分泌到培养基中的转化酶中轻转化酶的比例与与细胞结合的转化酶中轻转化酶的比例之比。有人提出这样的假说：在渗透突变体中以及在较小程度上在其他突变体中观察到的转化酶分泌增加和分离程度降低，可以用细胞壁孔隙率增加来解释。
