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与粗糙脉孢菌胞外酶超量产生相关的细胞壁改变

Cell wall alterations associated with the hyperproduction of extracellular enzymes in Neurospora crassa.

作者信息

Gratzner H G

出版信息

J Bacteriol. 1972 Aug;111(2):443-6. doi: 10.1128/jb.111.2.443-446.1972.

Abstract

A pleiotropic mutation in Neurospora (exo-1), which confers derepression of alpha-amylase, glucoamylase, beta-fructofuranosidase, and trehalase, appears to also affect the composition of the cell wall. Segregants resulting from the backcross of exo-1 to the wild-type strain from which it derived are altered in the ratio of galactosamine to glucosamine in hydrolysates of isolated cell walls. Conidial cell walls exhibit a marked decrease in the amount of galactosamine in both exo-1 and exo-1(+) strains. Increased levels (approximately sevenfold) of amylase are found in conidia of exo-1, as compared with those of exo-1(+).

摘要

粗糙脉孢菌中的一种多效性突变(exo - 1)可导致α - 淀粉酶、葡糖淀粉酶、β - 呋喃果糖苷酶和海藻糖酶的去阻遏,该突变似乎还会影响细胞壁的组成。exo - 1与其所源自的野生型菌株回交产生的分离子,其分离细胞壁水解产物中半乳糖胺与葡糖胺的比例发生了改变。在exo - 1和exo - 1(+)菌株的分生孢子细胞壁中,半乳糖胺的含量均显著降低。与exo - 1(+)相比,exo - 1分生孢子中的淀粉酶水平增加了(约7倍)。

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