Briziarelli G, Lusignani A, Pedrali C
Biochem J. 1969 Mar;112(1):35-9. doi: 10.1042/bj1120035.
The possible presence of steroids in the tissue of induced hormone-dependent rat mammary tumours was investigated. The method used involves a preliminary extraction of tumours followed by chemical separation and thin-layer chromatography. The identified compounds were cholesterol, androst-4-ene-3,17-dione, 5beta-androst-1-ene-3,17-dione, androsta-1,4-diene-3,17-dione and oestrone. This is the first report of the presence of these steroids in the tissue of an experimental tumour of a non-endocrine organ. In particular 5beta-androst-1-ene-3,17-dione has not previously been identified from natural sources.
对诱导的激素依赖性大鼠乳腺肿瘤组织中可能存在的类固醇进行了研究。所采用的方法包括先对肿瘤进行提取，然后进行化学分离和薄层色谱分析。鉴定出的化合物有胆固醇、雄甾-4-烯-3,17-二酮、5β-雄甾-1-烯-3,17-二酮、雄甾-1,4-二烯-3,17-二酮和雌酮。这是首次报道这些类固醇存在于非内分泌器官的实验性肿瘤组织中。特别是5β-雄甾-1-烯-3,17-二酮此前尚未从天然来源中鉴定出来。
