使用放射性同位素测定法检测病毒诱导酶活性的牛痘中和试验。
Vaccinia neutralization test using a radioisotope assay for virus-induced enzyme activity.
作者信息
Ziegler D W, Hutchinson H D
出版信息
Appl Microbiol. 1969 Jan;17(1):41-7. doi: 10.1128/am.17.1.41-47.1969.
Abstract
Vaccinia virus induction of a metabolic activity in host cell cultures forms the basis of a new assay for neutralizing antibodies. A direct relationship between the amount of vaccinia virus infecting cell cultures and the induced incorporation of tritium-labeled thymidine into the acid-insoluble fraction of the cells provided an indicator system. A liquid scintillation spectrometer was used to determine radioactivity associated with cell materials, and it provided a method for partial automation of an immunological procedure. Reproducibility of the method was satisfactory, and agreement with conventional vaccinia serum neutralization tests was demonstrated.
摘要
痘苗病毒在宿主细胞培养物中诱导代谢活性，构成了一种新型中和抗体检测方法的基础。感染细胞培养物的痘苗病毒量与诱导的氚标记胸腺嘧啶核苷掺入细胞酸不溶部分之间的直接关系提供了一种指示系统。使用液体闪烁光谱仪测定与细胞材料相关的放射性，它提供了一种使免疫程序部分自动化的方法。该方法的重现性令人满意，并证明与传统的痘苗血清中和试验结果一致。
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Vaccinia neutralization test using a radioisotope assay for virus-induced enzyme activity.使用放射性同位素测定法检测病毒诱导酶活性的牛痘中和试验。
Appl Microbiol. 1969 Jan;17(1):41-7. doi: 10.1128/am.17.1.41-47.1969.
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引用本文的文献
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Micro radiometric method for study of acid-insoluble materials in monolayer cell cultures.用于研究单层细胞培养物中酸不溶性物质的微量放射测量法。
Appl Microbiol. 1969 Jan;17(1):34-40. doi: 10.1128/am.17.1.34-40.1969.
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Kinetics of the vaccinia virus plaque neutralization test.牛痘病毒蚀斑中和试验的动力学
Appl Microbiol. 1971 May;21(5):968-70. doi: 10.1128/am.21.5.968-970.1971.
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Titration of relative neutralizing potency of vaccinia immune serum in tube cultures of monkey kidney cells.在猴肾细胞的试管培养物中滴定牛痘免疫血清的相对中和效力。
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DNA polymerase and deoxyribonucleotide kinase activities in cells infected with vaccinia virus.感染痘苗病毒的细胞中的DNA聚合酶和脱氧核糖核苷酸激酶活性。
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DELETION OF THYMIDINE KINASE ACTIVITY FROM L CELLS RESISTANT TO BROMODEOXYURIDINE.从对溴脱氧尿苷有抗性的L细胞中删除胸苷激酶活性。
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Induction of thymidine kinase by vaccinia-infected mouse fibroblasts.痘苗病毒感染的小鼠成纤维细胞诱导胸苷激酶的产生。
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Acquisition of thymidine kinase activity by herpes simplex-infected mouse fibroblast cells.单纯疱疹感染的小鼠成纤维细胞获得胸苷激酶活性。
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