用于研究单层细胞培养物中酸不溶性物质的微量放射测量法。
Micro radiometric method for study of acid-insoluble materials in monolayer cell cultures.
作者信息
Ziegler D W, Hutchinson H D, Palmer E L
出版信息
Appl Microbiol. 1969 Jan;17(1):34-40. doi: 10.1128/am.17.1.34-40.1969.
A simple radiometric procedure for study of acid-insoluble products synthesized in monolayer cell cultures is described. Cell cultures were produced directly on the bottom surface of scintillation vials or on glass cover slips (8 X 30 mm). The cells were labeled and extracted; the radioactivity was determined while the cells remained affixed to the glass surface upon which they were grown. This procedure enabled rapid investigations of certain biosynthetic processes to be carried out by using many individual cell cultures. The method was applied to an investigation of (3)H-thymidine incorporation induced by vaccinia virus in a 5-bromodeoxyuridine-resistant cell line. (14)C-labeling was evaluated as an alternate procedure for cell quantitation.
本文描述了一种用于研究单层细胞培养物中合成的酸不溶性产物的简单放射性测量方法。细胞培养物直接在闪烁瓶的底面或玻璃盖玻片（8×30毫米）上产生。对细胞进行标记和提取；在细胞仍附着于其生长的玻璃表面时测定放射性。该方法能够通过使用许多单独的细胞培养物对某些生物合成过程进行快速研究。该方法应用于痘苗病毒在5-溴脱氧尿苷抗性细胞系中诱导的（3）H-胸苷掺入的研究。（14）C标记作为细胞定量的替代方法进行了评估。
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