Shtasel T F, Berry L J
J Bacteriol. 1969 Mar;97(3):1018-25. doi: 10.1128/jb.97.3.1018-1025.1969.
The effect of cortisone and endotoxin, singly and in combination, on ribonucleic acid (RNA) synthesis in livers of adrenalectomized mice was determined. This was accomplished by measuring the incorporation either of inorganic (32)P or of (14)C-orotic acid into the RNA. Under similar conditions, the effect of these agents on the rate of protein synthesis was examined with the use of (14)C-leucine. Bacterial endotoxin was found to augment the uptake of isotope in the RNA and in the protein of the liver. These reactions did not appear to be mediated via the pancreatic hormone insulin, which was found to depress the incorporation of the radioactive compounds into RNA. Cortisone increased the uptake of isotope in liver RNA but depressed the incorporation of leucine into hepatic protein. These results indicate that the previously observed ability of endotoxin to prevent the hormone induction of hepatic enzymes, such as tryptophan oxygenase, is not associated with impaired synthesis of liver RNA or protein.
研究了可的松和内毒素单独及联合使用对肾上腺切除小鼠肝脏中核糖核酸（RNA）合成的影响。这是通过测量无机（32）P或（14）C - 乳清酸掺入RNA来实现的。在类似条件下，使用（14）C - 亮氨酸研究了这些药物对蛋白质合成速率的影响。发现细菌内毒素可增加肝脏RNA和蛋白质中同位素的摄取。这些反应似乎不是通过胰腺激素胰岛素介导的，胰岛素被发现会抑制放射性化合物掺入RNA。可的松增加了肝脏RNA中同位素的摄取，但降低了亮氨酸掺入肝脏蛋白质的量。这些结果表明，先前观察到的内毒素阻止激素诱导肝酶（如色氨酸加氧酶）的能力与肝脏RNA或蛋白质合成受损无关。
