Sedláková E, Lichardus B, Cort J H
Science. 1969 May 2;164(3879):580-2. doi: 10.1126/science.164.3879.580.
Plasma natriuretic activity was evoked in cows and dogs by infusion of saline with or without dextran. Deproteinized samples were fractionated on both Sephadex and Bio-Gel columns; the activity was separated, the approximate molecular weight being in the region of 1000. Incubation with chymotrypsin destroyed the activity, suggesting that it might be a polypeptide. A similar activity in blood resulted from intracarotid injection of either oxytocin or either of two synthetic analogs. Possibly the latter are saluretic by virtue of a releasing action on some intracranial structure for another natriuretic peptide.
通过输注含或不含右旋糖酐的生理盐水，可在奶牛和狗体内诱发血浆利钠活性。对脱蛋白样品在葡聚糖凝胶柱和生物凝胶柱上进行分级分离；活性物质被分离出来，其近似分子量在1000左右。用胰凝乳蛋白酶孵育会破坏该活性，表明它可能是一种多肽。颈内注射催产素或两种合成类似物中的任何一种，均可在血液中产生类似活性。可能后者具有促尿钠排泄作用，是因为它对另一种利钠肽的某些颅内结构具有释放作用。
