Kerwin R M, Ruelius H W
Appl Microbiol. 1969 Mar;17(3):347-51. doi: 10.1128/am.17.3.347-351.1969.
An Amber MPH-Cerelose medium was devised on which the unidentified basidiomycete B 191039 produced high titers of alcohol oxidase in shake cultures. Another, fully synthetic medium was developed on which the production was nearly as good, but very slow. No cofactors were required to produce this flavine adenine dinucleotide-containing enzyme. The B 191039 organism underwent frequent mutations. The mutant forms were morphologically distinct from the parent strain and elaborated little or no alcohol oxidase. Several other basidiomycetes were shown to produce alcohol oxidase in submerged culture.
设计了一种琥珀色MPH-糊精培养基，在该培养基上，未鉴定的担子菌B 191039在摇瓶培养中产生高滴度的乙醇氧化酶。还开发了另一种完全合成培养基，在该培养基上产量几乎相同，但非常缓慢。产生这种含黄素腺嘌呤二核苷酸的酶不需要辅因子。B 191039菌株经常发生突变。突变形式在形态上与亲本菌株不同，产生很少或不产生乙醇氧化酶。其他几种担子菌在深层培养中也能产生乙醇氧化酶。
