Production of alcohol oxidase by several Basidiomycetes.

An Amber MPH-Cerelose medium was devised on which the unidentified basidiomycete B 191039 produced high titers of alcohol oxidase in shake cultures. Another, fully synthetic medium was developed on which the production was nearly as good, but very slow. No cofactors were required to produce this flavine adenine dinucleotide-containing enzyme. The B 191039 organism underwent frequent mutations. The mutant forms were morphologically distinct from the parent strain and elaborated little or no alcohol oxidase. Several other basidiomycetes were shown to produce alcohol oxidase in submerged culture.