儿童血清生长激素放射受体分析与放射免疫分析的相关性研究：正常儿童与生长激素治疗的垂体性侏儒症患儿

Correlative study of radioreceptor assay and radioimmunoassay of serum growth hormone in children: normal children and HGH-treated pituitary dwarfs.

作者信息

Garnier P E, Job J C

出版信息

Acta Endocrinol (Copenh). 1977 Sep;86(1):50-9. doi: 10.1530/acta.0.0860050.

DOI:10.1530/acta.0.0860050

Abstract

A sensitive and reproducible radioreceptor assay (RRA) for human growth hormone (HGH) is described. It allows the evaluation of HGH concentrations as low as 2 ng/ml. It has a limited cross-reactivity with human prolactin, which does not interfere at physiological levels in children. Comparison of the results with those of radioimmunoassay (RIA) showed no discrepancies in the serum of normal children before and after stimulation tests for GH (mean RRA/RIA ratio 1.03 +/- SEM 0.04, range 0.75 to 1.65) nor in the serum from hypopituitary dwarfs during the 12 h following an im injection of 6 mg of HGH (mean RRA/RIA ratio 1.05 +/- 0.04, range 0.84 to 1.28). It is concluded that receptoractivity of HGH is parallel to its immunoreactivity in normal children and in hypopituitary patients clinical grade HGH.

摘要

本文描述了一种用于检测人生长激素（HGH）的灵敏且可重复的放射受体分析方法（RRA）。该方法能够检测低至2 ng/ml的HGH浓度。它与人催乳素的交叉反应有限，在儿童生理水平下不会产生干扰。将该方法的结果与放射免疫分析（RIA）的结果进行比较，发现在对正常儿童进行GH刺激试验前后的血清中（RRA/RIA平均比值为1.03±标准误0.04，范围为0.75至1.65），以及在皮下注射6 mg HGH后的12小时内，垂体功能减退性侏儒症患者的血清中（RRA/RIA平均比值为1.05±0.04，范围为0.84至1.28），两者结果均无差异。由此得出结论，在正常儿童和垂体功能减退患者的临床级HGH中，HGH的受体活性与其免疫活性平行。