Schroder H D, Sperling S
Acta Ophthalmol (Copenh). 1977 Oct;55(5):819-26. doi: 10.1111/j.1755-3768.1977.tb08281.x.
Electron microscopy revealed the presence of a 600-1500 A thick layer of polysaccharide on the surface of human corneal endothelial cells. The surface layer was visualized by combined fixation and staining in a mixture of ruthenium red and osmium tetroxide. The coating material was stable for at least 39 h post mortem and was retained on disintegrating cells.
电子显微镜检查显示,在人角膜内皮细胞表面存在一层600 - 1500埃厚的多糖层。通过在钌红和四氧化锇的混合物中进行联合固定和染色,可观察到该表面层。这种涂层材料在死后至少39小时内保持稳定,并保留在正在解体的细胞上。