采用带电子捕获检测的气相色谱法同时测定生物样品中的可待因和吗啡。

Simultaneous determination of codeine and morphine in biological samples by gas chromatography with electron capture detection.

作者信息

Dahlström B, Paalzow L, Edlund P O

出版信息

Acta Pharmacol Toxicol (Copenh). 1977 Sep;41(3):273-9. doi: 10.1111/j.1600-0773.1977.tb02148.x.

DOI:10.1111/j.1600-0773.1977.tb02148.x

Abstract

A sensitive gas chromatographic method for the simultaneous determination of codeine and morphine in plasma and brain samples is described. The method involves solvent extraction of the compounds from plasma, derivatization with pentafluoropropionic anhydride and subsequent separation on a 3% OV-17 column. The quantification is performed with electron capture detection. The sensitivity of the method (0.75 ng of morphine and 7.5 ng of codeine in a sample) makes it especially useful for pharmacokinetic investigations. The method was successfully applied to determine the time course of codeine and its metabolite morphine after intravenous administration of codeine to the rat.

摘要

本文描述了一种用于同时测定血浆和脑样本中可待因和吗啡的灵敏气相色谱法。该方法包括从血浆中溶剂萃取化合物，用五氟丙酸酐进行衍生化，随后在3% OV-17柱上进行分离。定量采用电子捕获检测法。该方法的灵敏度（样品中0.75 ng吗啡和7.5 ng可待因）使其在药代动力学研究中特别有用。该方法已成功应用于测定大鼠静脉注射可待因后可待因及其代谢产物吗啡的时间进程。