大鼠大脑皮层中乙酰胆碱合成与其浓度之间的关系。
Relationship between acetylcholine synthesis and its concentration in rat cerebral cortex.
作者信息
Sharkawi M, Schulman M P
出版信息
Br J Pharmacol. 1969 Jun;36(2):373-9. doi: 10.1111/j.1476-5381.1969.tb09512.x.
Abstract
- The synthesis of carbon-14 labelled acetylcholine ((14)C-ACh) from carbon-14 uniformly labelled glucose (U.L. (14)C-glucose) under different conditions has been studied.2. Increasing KCl concentration from 4 mM to 31 mM was associated with a large increase in the amount of (14)C-ACh in the medium and a significant decrease in the amount of (14)C-ACh in the tissue. This effect was observed in slices and minces but not in homogenate.3. Increasing KCl concentration from 4 mM to 31 mM resulted in the release of pre-formed (14)C-ACh.4. Elevated pre-incubation levels of ACh in slices incubated in 4 mM KCl medium was associated with a decrease in the formation of (14)C-ACh.5. These findings are consistent with the hypothesis that the concentration of ACh in the vicinity of the site of synthesis regulates the rate of ACh formation.
摘要
研究了在不同条件下，由碳-14均匀标记的葡萄糖（U.L.（14）C-葡萄糖）合成碳-14标记的乙酰胆碱（（14）C-ACh）的情况。
将氯化钾浓度从4 mM提高到31 mM，培养基中（14）C-ACh的量大幅增加，而组织中（14）C-ACh的量显著减少。在切片和碎末中观察到了这种效应，但在匀浆中未观察到。
将氯化钾浓度从4 mM提高到31 mM导致预先形成的（14）C-ACh释放。
在4 mM氯化钾培养基中孵育的切片中，乙酰胆碱预孵育水平升高与（14）C-ACh形成减少有关。
这些发现与以下假设一致，即合成部位附近的乙酰胆碱浓度调节乙酰胆碱的形成速率。
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引用本文的文献
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本文引用的文献
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J Physiol. 1936 Mar 9;86(3):290-305. doi: 10.1113/jphysiol.1936.sp003364.
2
The spontaneous release of acetylcholine from the denervated hemidiaphragm of the rat.大鼠去神经支配半膈肌中乙酰胆碱的自发释放。
J Physiol. 1963 Jan;165(1):117-29. doi: 10.1113/jphysiol.1963.sp007046.
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Biochem J. 1939 May;33(5):822-35. doi: 10.1042/bj0330822.
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