Scheres J M, Merkx G F
Hum Genet. 1976 May 19;32(2):155-69. doi: 10.1007/BF00291499.
In this paper a technique is described for the banding of human metaphase chromosomes with basic fuchsin. The main characteristics of the G-banding pattern obtained with this cationic triphenylmethane dye are: the secondary constriction regions of chromosomes Nos. 1 and 16 are strongly stained, especially in the latter one; the heterochromatic area of chromosome No. 9, usually negative with most other G-banding techniques, is clearly visible as an intensely stained band adjacent to the centromere; the chromosomal outline is often very distinct, facilitating the study of the telomeres; a number of chromosomal regions with bright Q fluorescence such as the polymorphic regions of the chromosomes Nos. 3, 4, and Y also stain strongly with basic fuchsin. The basic fuchsin technique combines therefore properties of G-, C-, and Q-banding methods and seems very suitable for use in e.g., family and linkage studies. Several triphenylmethanes closely related to basic fuchsin produce similar banding patterns. The band-producing ability is, however, diminished in those dyes which contain methylated amino groups. If the methyl groups are attached to the carbon atoms at the 3-positions in the phenyl rings, band formation seems unaffected. The way in which basic fuchsin and chromatin may interact as well as the possible mechanism(s) of band formation with this dye are discussed.
本文描述了一种用碱性品红对人类中期染色体进行显带的技术。用这种阳离子三苯甲烷染料获得的G带模式的主要特征是：1号和16号染色体的次缢痕区域染色强烈，尤其是后者；9号染色体的异染色质区，在大多数其他G带技术中通常呈阴性，在靠近着丝粒处可见一条强烈染色的带，清晰可见；染色体轮廓通常非常清晰，便于端粒的研究；一些具有明亮Q荧光的染色体区域，如3号、4号和Y染色体的多态性区域，也能用碱性品红强烈染色。因此，碱性品红技术结合了G带、C带和Q带方法的特性，似乎非常适合用于例如家族和连锁研究。几种与碱性品红密切相关的三苯甲烷产生类似的带型模式。然而，那些含有甲基化氨基的染料的显带能力会降低。如果甲基连接在苯环3位的碳原子上，带的形成似乎不受影响。本文讨论了碱性品红与染色质相互作用的方式以及用这种染料形成带的可能机制。
