Scheres J M, Merkx G F, Hustinx T W
Hum Genet. 1982;61(1):8-11. doi: 10.1007/BF00291322.
A technique is described for the production of detailed and richly contrasting G-band patterns in human prometaphase chromosomes with the aid of the triphenylmethane dye basic fuchsin. The usefulness of this method is illustrated by its application for the precise analysis of two chromosome 11 rearrangements. It is also demonstrated that high-resolution banding with basic fuchsin can reveal bands not present in the international standard idiogram of human prophase chromosomes (ISCN 1981). The technique described can also be used for easy recognition of the late replicating X chromosome, which stains darker than its early replicating homologue. A preliminary analysis of the late replicating X chromosomes in a 49,XXXXY individual suggests that the three supernumerary X chromosomes do not necessarily replicate synchronously.
本文描述了一种借助三苯甲烷染料碱性品红在人类早中期染色体上产生详细且对比度丰富的G带模式的技术。通过将该方法应用于两条11号染色体重排的精确分析，说明了此方法的实用性。还证明了用碱性品红进行高分辨率显带可揭示人类前期染色体国际标准核型图（ISCN 1981）中不存在的带。所描述的技术还可用于轻松识别晚复制的X染色体，其染色比早复制的同源染色体更深。对一名49,XXXXY个体中晚复制X染色体的初步分析表明，三条额外的X染色体不一定同步复制。
