Bibbiani C, Tongiani R, Viola-Magni M P
J Cell Biol. 1969 Aug;42(2):444-51. doi: 10.1083/jcb.42.2.444.
A method for the determination of the DNA content of isolated nuclei of different ploidy has been developed. It is based on measurement of the nuclear dry mass, with an integrating microinterferometer, before and after DNase treatment. The values found are slightly low, because, as indicated by biochemical determinations, consistently 5% to 8% of DNA is not extracted by DNase under these conditions. The average DNA values thus obtained for diploid and tetraploid nuclei of adult rat liver are 7.7 and 15.6 pg (10(-12) g), respectively. Definite advantages of this procedure are: i) comparisons with biochemical determinations to give DNA values for each class of ploidy, ii) comparisons with histophotometry of the Feulgen dye-DNA complex to give absolute values instead of arbitrary units.
已开发出一种用于测定不同倍性分离细胞核DNA含量的方法。该方法基于在DNase处理前后，使用积分式显微干涉仪测量细胞核干质量。所得到的值略低，因为如生化测定所示，在这些条件下，始终有5%至8%的DNA不能被DNase提取。由此获得的成年大鼠肝脏二倍体和四倍体细胞核的平均DNA值分别为7.7和15.6皮克（10^(-12)克）。该方法的明确优点是：i）与生化测定进行比较，以得出每类倍性的DNA值；ii）与Feulgen染料-DNA复合物的组织光度测定进行比较，以得出绝对值而非任意单位。
