Bereiter-Hahn J, Wientzeck C, Bröhl H
Histochemistry. 1981;73(2):269-84. doi: 10.1007/BF00493026.
A population of endothelial cells grown from Xenopus laevis tadpole hearts was investigated by microinterferometry. The resulting interferograms were evaluated by an automatic image analyzer. The mean values of dry mass were 778 +/- 340 pg (10(-15) g) for whole cells, 648 +/- 309 pg for cytoplasm, 116 +/- 45 pg for nuclei, and 19 +/- 10 pg for nucleoli. Two subpopulations of cells were identified, an actively growing one and a less active one. The density (dry mass per microm2) of the nuclei and nucleoli of less active cells was greater than that of the nuclei and nucleoli of actively growing cells. In addition, the inactive cells were always large and possessed a considerable amount of cytoplasm. The entrance of cells into S-phase could not be detected by microinterferometry; and no differences were apparent between cells possessing one nucleolus and those containing two nucleoli. The values obtained in these amphibian cells were compared with those derived from mammalian cells.
利用显微干涉测量法对非洲爪蟾蝌蚪心脏来源的内皮细胞群体进行了研究。通过自动图像分析仪对所得干涉图进行评估。全细胞干质量的平均值为778±340皮克（10⁻¹⁵克），细胞质为648±309皮克，细胞核为116±45皮克，核仁为19±10皮克。鉴定出了两个细胞亚群，一个是活跃生长的亚群，另一个是活性较低的亚群。活性较低细胞的细胞核和核仁的密度（每平方微米干质量）大于活跃生长细胞的细胞核和核仁的密度。此外，不活跃细胞总是较大，且拥有大量细胞质。显微干涉测量法无法检测到细胞进入S期；拥有一个核仁的细胞与含有两个核仁的细胞之间没有明显差异。将这些两栖类细胞获得的值与源自哺乳动物细胞的值进行了比较。
