Brandchaft P B, Aoki T, Silverman T N
Int J Cancer. 1976 May 15;17(5):678-85. doi: 10.1002/ijc.2910170518.
Soluble extracts from Moloney virus-induced leukemia cells were shown to contain immunogenic and immunosuppressive components. Immunogenic components were divided into two classes based on their ability to :(1) combine with humoral antibody to inhibit cytotoxic activity; or (2) stimulate blastogenesis in normal syngeneic lymphocytes. Immunosuppressive components were demonstrated by the inhibition of DNA synthesis in normal syngeneic lymphocytes and suppression of a mixed lymphocyte reaction. Stimulating components in many soluble preparations were completely masked by immunosuppressive components. However, all three components could be resolved by Sephadex G200 chromatography.
莫洛尼病毒诱导的白血病细胞的可溶性提取物被证明含有免疫原性和免疫抑制成分。免疫原性成分根据其能力分为两类：（1）与体液抗体结合以抑制细胞毒性活性；或（2）刺激正常同基因淋巴细胞的胚细胞生成。免疫抑制成分通过抑制正常同基因淋巴细胞中的DNA合成和抑制混合淋巴细胞反应来证明。许多可溶性制剂中的刺激成分被免疫抑制成分完全掩盖。然而，所有这三种成分都可以通过葡聚糖G200层析分离。
