Rooney S A, Gobran L I
Lipids. 1977 Dec;12(12):1050-4. doi: 10.1007/BF02533333.
Pulmonary surfactant, the major surface-active component of which in the adult is dipalmitoylglycerophosphocholine, can be obtained by lavaging lungs with physiological saline. We have previously shown that there is an increase in the amount of phosphatidylcholine in fetal rabbit lung lavage during the latter part of gestation. We have now measured the amount of disaturated phosphatidylcholine as well as the fatty acid composition of phosphatidylcholine in lung lavage from fetal rabbits during the period 27 days' gestation to full term (31 days). There was no developmental change in the amount of disaturated phosphatidylcholine during the period examined. About 50% of the total phosphatidylcholine was disaturated which is approximately the same as in adult rabbit lung lavage. The fatty acid composition, however, did change. There was an increase in the amount of 16:0 from about 20% of the total fatty acids in phosphatidylcholine at 27-28 days to about 60% at full term, after birth, and in the adults. There was a corresponding decrease in the amounts of 14:0, 18:0, and longer chain fatty acids, most of which were saturated. In the lavaged lung tissue, there was a 2.6-fold increase in the percentage of phosphatidylcholine which was disaturated during the period 27-31 days' gestation. It had not decrease to the adult value 24 hr after birth.
肺表面活性物质，其在成人中的主要表面活性成分是二棕榈酰甘油磷酸胆碱，可以通过用生理盐水灌洗肺部来获得。我们之前已经表明，在妊娠后期，胎兔肺灌洗中磷脂酰胆碱的量会增加。我们现在测量了从妊娠27天到足月（31天）期间胎兔肺灌洗中双饱和磷脂酰胆碱的量以及磷脂酰胆碱的脂肪酸组成。在所检查的期间内，双饱和磷脂酰胆碱的量没有发育变化。约50%的总磷脂酰胆碱是双饱和的，这与成年兔肺灌洗中的情况大致相同。然而，脂肪酸组成确实发生了变化。16:0的量从妊娠27 - 28天时磷脂酰胆碱中总脂肪酸的约20%增加到足月、出生后以及成年时的约60%。14:0、18:0和更长链脂肪酸（其中大多数是饱和的）的量相应减少。在灌洗的肺组织中，妊娠27 - 31天期间双饱和磷脂酰胆碱的百分比增加了2.6倍。出生后24小时它并未降至成年值。
