Cecilia M, Rost M, Rost F W
Histochem J. 1976 Jan;8(1):93-8. doi: 10.1007/BF01004010.
Masked metachromasia is demonstrated by staining with a metachromatic basic dye, after acid hydrolysis of suitably fixed tissue. We report that the addition of 20% Carbowax 20M (an inert polyer, mol. wt. about 20,000) to the hydrolysis mixture improved the reaction. The improved method gives increased metachromasia , greater tolerance to variations in hydrolysis conditions, and demonstrates a greater proportion of cells--presumably due to a lower threshold of sensitivity. Lower molecular weight polymers (Carbowax 1000, Carbowax 6000) are less effective.
在对适当固定的组织进行酸水解后，用一种异染性碱性染料染色可显示出隐蔽异染性。我们报告称，在水解混合物中添加20%的聚乙二醇20M（一种惰性聚合物，分子量约为20,000）可改善反应。改进后的方法可提高异染性，对水解条件变化的耐受性更强，并且能显示出更大比例的细胞——这可能是由于较低的敏感性阈值所致。较低分子量的聚合物（聚乙二醇1000、聚乙二醇6000）效果较差。
