DeLellis R A, Balogh K
Am J Pathol. 1973 Jul;72(1):119-28.
Normal canine parafollicular cells share a common set of histochemical characteristics, which include masked metachromasia and argyrophilia, with human as well as rat medullary thyroid carcinomas. Masked metachromasia, a property of polypeptide hormone-producing cells, was demonstrated by staining with toluidine blue or coriophosphine O after acid hydrolysis. Argyrophilia was demonstrated both by the Grimelius silver nitrate technic and by the Azzopardi modification of the Bodian method. With these technics, parafollicular cells in the dog and medullary carcinoma of human and rat origin showed nearly identical staining reactions. Fixation in glutaraldehyde was superior to formaldehyde in demonstrating masked metachromasia of normal dog parafollicular cells, while formaldehyde fixation was superior for the demonstration of argyrophilia of granules. In general, the Grimelius method was superior to the Azzopardi modification of the Bodian technic for the demonstration of argyophilia. The results of this study provided further support for the parafollicular cell origin of human and rat medullary thyroid carcinomas and also provided a useful set of histochemical criteria for the diagnosis of this neoplasm.
正常犬的滤泡旁细胞与人类以及大鼠的甲状腺髓样癌具有一组共同的组织化学特征，包括隐蔽性异染性和嗜银性。隐蔽性异染性是产生多肽激素细胞的一种特性，通过酸水解后用甲苯胺蓝或考里磷光素O染色来证明。嗜银性通过格里梅利乌斯硝酸银技术以及博迪安方法的阿佐帕迪改良法来证明。使用这些技术，犬的滤泡旁细胞以及人类和大鼠来源的甲状腺髓样癌显示出几乎相同的染色反应。在显示正常犬滤泡旁细胞的隐蔽性异染性方面，戊二醛固定优于甲醛固定，而甲醛固定在显示颗粒的嗜银性方面更优。一般来说，在显示嗜银性方面，格里梅利乌斯方法优于博迪安技术的阿佐帕迪改良法。本研究结果为人类和大鼠甲状腺髓样癌的滤泡旁细胞起源提供了进一步支持，也为该肿瘤的诊断提供了一组有用的组织化学标准。
