Edozien J C, Switzer B R
J Nutr. 1978 Feb;108(2):282-90. doi: 10.1093/jn/108.2.282.
Twenty-eight-day old male Sprague Dawley rats were fed, either ad libitum or in restricted amounts, isoenergetic diets containing 2%, 5%, 10%, 15%, 25%, or 50% lactalbumin protein and 5%, 11.9%, or 21.1% fat for 8 weeks and were then killed. Weekly food consumption and body weight, terminal weight, body water and lipid, and liver weight, DNA, RNA, protein, and lipid were measured. The growth rate increased progressively with each increase in the level of dietary protein up to 25% protein and then declined. Growth was also accelerated by a high fat diet but was retarded by restriction of energy intake. Total body lipid correlated directly with the level of fat in the diet. Multiple regression analysis of the type: Y = beta0 + beta1X1 + beta2X2 + B3X3 + B4X4 where Y = rate of weight gain X1 = dietary protein level, X2 = protein efficiency ratio, X3 = appetite factor, and X4 = energy/protein ratio, showed that the maximum rate of weight gain of 58.8 g/week occurred when the diet contained 23% protein. Growth rate declined when the diet contained a higher protein level.
给28日龄的雄性斯普拉格-道利大鼠随意或限量喂食等能量日粮，日粮含2%、5%、10%、15%、25%或50%的乳白蛋白以及5%、11.9%或21.1%的脂肪，持续8周，然后将其处死。测量每周的食物消耗量、体重、终末体重、体内水分和脂质以及肝脏重量、DNA、RNA、蛋白质和脂质。随着日粮蛋白质水平提高至25%，生长速率逐渐增加，之后下降。高脂日粮也加速生长，但能量摄入受限则生长迟缓。体内总脂质与日粮中的脂肪水平直接相关。对Y = beta0 + beta1X1 + beta2X2 + B3X3 + B4X4这种类型进行多元回归分析，其中Y = 体重增加速率，X1 = 日粮蛋白质水平，X2 = 蛋白质效率比，X3 = 食欲因子，X4 = 能量/蛋白质比，结果显示当日粮含23%蛋白质时，体重增加的最大速率为58.8克/周。当日粮蛋白质水平更高时，生长速率下降。
