通过免疫扩散和感染后中和试验研究甲型流感病毒神经氨酸酶（N2）抗原之间的抗原关系。
Antigenic relationships among influenza virua A neuraminidase (N2) antigens by immunodiffusion and postinfection neutralization tests.
作者信息
Dowdle W R, Laver W G, Galphin J C, Downie J C
出版信息
J Clin Microbiol. 1976 Mar;3(3):233-8. doi: 10.1128/jcm.3.3.233-238.1976.
The antigenic relationships among the neuraminidases of influenza A strains from 1957 to 1973 were examined by postinfection application of neuraminidase antisera. This procedure causes inhibition of virus spread and apparent neutralization. Neuraminidase (apparent) neutralization and neuraminidase inhibition tests with chicken antisera gave similar results. Neuraminidase inhibition tests were more discriminating than neuraminidase neutralization tests when rabbit and goat antisera were used. Antibody absorption studies revealed that the neuraminidase, like the hemagglutinin, may possess two kinds of antigenic determinants, which can give rise to "common," or "cross-reacting," and "specific" antibodies. "Specific" antibody appears to be more effective in the inhibition of enzyme activity than in the inhibition of virus spread.
通过感染后应用神经氨酸酶抗血清，检测了1957年至1973年甲型流感病毒株神经氨酸酶之间的抗原关系。该程序可抑制病毒传播并实现明显的中和作用。用鸡抗血清进行的神经氨酸酶（表观）中和试验和神经氨酸酶抑制试验结果相似。当使用兔和山羊抗血清时，神经氨酸酶抑制试验比神经氨酸酶中和试验更具区分性。抗体吸收研究表明，神经氨酸酶与血凝素一样，可能具有两种抗原决定簇，可产生“共同”或“交叉反应”抗体以及“特异性”抗体。“特异性”抗体在抑制酶活性方面似乎比抑制病毒传播更有效。
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