Pariza M W, Iandolo J J
Appl Microbiol. 1969 Jun;17(6):836-8. doi: 10.1128/am.17.6.836-838.1969.
Suspensions of Staphylococcus aureus MF-31 injured by heat treatment at 54 C for 15 min produced coagulase during recovery in Trypticase Soy Broth. Coagulase also was produced by injured cells during recovery in a medium that did not support growth. Coagulase synthesis during recovery was independent of the molar strength of the buffer in which the cells were injured, the age of the cells, and the degree of injury. Return of salt tolerance and coagulase production required glucose, amino acids, and phosphate in the recovery medium. Vitamins stimulated coagulase production, but did not affect recovery. Although coagulase production was not necessary for repair of thermal injury to S. aureus MF-31, its detection was interpreted as an indicator of protein synthesis.
在54℃热处理15分钟而受损的金黄色葡萄球菌MF-31悬液，在胰蛋白酶大豆肉汤中复苏期间产生了凝固酶。在不支持生长的培养基中复苏时，受损细胞也产生了凝固酶。复苏期间的凝固酶合成与细胞受损时所处缓冲液的摩尔浓度、细胞菌龄以及损伤程度无关。恢复耐盐性和产生凝固酶需要复苏培养基中有葡萄糖、氨基酸和磷酸盐。维生素刺激了凝固酶的产生，但不影响复苏。虽然凝固酶的产生对于金黄色葡萄球菌MF-31热损伤的修复并非必要，但其检测被视为蛋白质合成的一个指标。
