热损伤金黄色葡萄球菌细胞恢复过程中的核糖体组装
Ribosome assembly during recovery of heat-injured Staphylococcus aureus cells.
作者信息
Flowers R S, Martin S E
出版信息
J Bacteriol. 1980 Feb;141(2):645-51. doi: 10.1128/jb.141.2.645-651.1980.
The process of ribosome formation during repair of sublethal heat injury was examined in Staphylococcus aureus. Sublethal heating of this organism results in the degradation of the 30S ribosomal subunit and alteration of the 50S subunit. Cells recovering from sublethal injury were examined for changes with time in the sedimentation and electrophoretic properties of ribonucleoprotein particles and ribonucleic acid, respectively. When cells were allowed to recover in [3H]uridine, the label could be followed into ribonucleic acid species that coelectrophoresed with 23S and 16S ribonucleic acid. Three ribonucleoprotein particles (49S, 36S, and 30S) were isolated from repairing cells by sedimentation through sucrose gradients. Polyacrylamide gel electrophoresis showed that the 49S particle contained 23S ribonucleic acid, the 36S particle contained both 23S ribonucleic acid and 16S precursor and mature ribonucleic acid, and the 30S particle contained 16S and precursor 16S ribonucleic acid. Particles with similar sedimentation properties were found in unheated cells.
在金黄色葡萄球菌中研究了亚致死热损伤修复过程中的核糖体形成过程。该生物体的亚致死加热导致30S核糖体亚基降解和50S亚基改变。分别检查从亚致死损伤中恢复的细胞中核糖核蛋白颗粒和核糖核酸的沉降及电泳特性随时间的变化。当细胞在[3H]尿苷中恢复时，该标记可追踪到与23S和16S核糖核酸共电泳的核糖核酸种类中。通过蔗糖梯度沉降从修复细胞中分离出三种核糖核蛋白颗粒（49S、36S和30S）。聚丙烯酰胺凝胶电泳显示，49S颗粒含有23S核糖核酸，36S颗粒含有23S核糖核酸以及16S前体和成熟核糖核酸，30S颗粒含有16S和前体16S核糖核酸。在未加热的细胞中发现了具有相似沉降特性的颗粒。
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