Matthiaschk G, Roll R
Arch Toxicol. 1977 Nov 21;38(4):261-74. doi: 10.1007/BF00352031.
The acute LD50 in virginal NMRI-Mice was found to be 2528 mg/kg for monolinuron and 1791 mg/kg for buturon. Pregnant female mice of the NMRI strain were administered orally 25--1000 mg monolinuron/kg (I) and 100-400 mg buturon/kg (II) on days 6--15 of gestation and, during defined phases of fetal development (days 10--13 after conception), 500 mg I/kg and 350 mg II/kg. Following administration of 10 doses, an increase of postimplantative losses and clear retardation of development in the upper dose range from 100 mg I/kg and 300 mg II/kg as well as a dose-dependent increase of the rate of cleft palates could be observed. High doses of both substances given from day 6--15 of pregnancy produced minor numbers of wavy and fused ribs as well as hypoplasia of the upper jaw after application of monolinuron and exenteria and exencephaly after buturon. Administration of monolinuron between days 10 and 13 of gestation resulted a minor and that of buturon a clear increase of the number of cleft palates. To evaluate postnatal development 200 and 500 mg I/kg, and 200 and 300 mg II/kg were administered orally on days 6--15 of gestation. In the higher doses, both substances produced an increased mortality among the offspring up to 3 weeks after birth, and a clear increase of the rate of cleft palates.
对于纯处女NMRI小鼠，灭草隆的急性半数致死剂量（LD50）为2528毫克/千克，环草隆为1791毫克/千克。在妊娠第6至15天，给NMRI品系的怀孕雌性小鼠经口灌胃25 - 1000毫克灭草隆/千克（I组）和100 - 400毫克环草隆/千克（II组），并在胎儿发育的特定阶段（受孕后第10至13天），给予500毫克I组/千克和350毫克II组/千克。给药10次后，可观察到在100毫克I组/千克和300毫克II组/千克及以上的高剂量范围内，着床后损失增加，发育明显迟缓，并且腭裂发生率呈剂量依赖性增加。在妊娠第6至15天给予两种物质的高剂量时，给予灭草隆后出现少量波浪状和融合肋骨以及上颌发育不全，给予环草隆后出现脏器外翻和无脑儿。在妊娠第10至13天给予灭草隆导致腭裂数量略有增加，而给予环草隆则导致腭裂数量明显增加。为评估产后发育情况，在妊娠第6至15天经口给予200和500毫克I组/千克，以及200和300毫克II组/千克。在较高剂量下，两种物质均导致出生后3周内后代死亡率增加，并且腭裂发生率明显增加。
