Gross N J
J Lab Clin Med. 1978 Mar;91(3):537-44.
A study of surfactant physiology and biochemistry was undertaken in mice who received varying doses of X-irradiation to the thorax. During the first 12 weeks after X-irradiation, the period which precedes radiation pneumonitis, there is an increase in the amount of DPC in both the lung and the alveolar surface lining layer. This increase begins about 7 days after irradiation, returns to control levels by 12 weeks, and coincides with a fall in the total number of AM's. Over-all synthesis of DPC in the lung and its transport to the alveolar surface, as determined by incorporation of 3H from glycerol and 14C from palmitate into PC in vivo, remained unchanged from control levels. These results may be explained in part by proliferation of type 2 pneumocytes following X-irradiation and by decreased removal of surfactant from the alveolar surface, possibly as a result of the depletion of the AM population. No changes in mechanical properties of the lung or in situ E tis or E surf occurred at this stage, nor were changes detected in the surface properties of the alveolar lavage fluid.
对接受不同剂量胸部X线照射的小鼠进行了表面活性剂生理学和生物化学研究。在X线照射后的前12周，即放射性肺炎出现之前的这段时间，肺和肺泡表面衬里层中的二棕榈酰卵磷脂（DPC）量增加。这种增加在照射后约7天开始，到12周时恢复到对照水平，并且与肺泡巨噬细胞（AM）总数的下降同时发生。通过体内甘油中的3H和棕榈酸中的14C掺入磷脂酰胆碱（PC）来确定，肺中DPC的总体合成及其向肺泡表面的转运与对照水平相比保持不变。这些结果部分可以通过X线照射后Ⅱ型肺细胞的增殖以及肺泡表面表面活性剂清除减少来解释，这可能是由于AM数量减少所致。在此阶段，肺的力学性能、原位弹性回缩率（E tis）或表面弹性回缩率（E surf）均未发生变化，肺泡灌洗液的表面性质也未检测到变化。
