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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Serafini-Fracassini A, Durward J J, Floreani L

Biochem J. 1969 Apr;112(2):167-72. doi: 10.1042/bj1120167.

A procedure for the isolation and purification of the heparin-protein complex from ox liver capsule, based on the solubility properties of mucopolysaccharide-cetylpyridinium complexes, is described. 2. The yield of the heparin-protein complex with this method averages 35mg./100g. of dry ox liver capsule. 3. The results of analyses on the polysaccharide show good agreement with values previously published for purified heparin fractions. 4. The amino acid analysis of the protein component shows several similarities to that of chondromucoprotein. 5. The results of beta-carbonyl elimination, either with or without catalytic hydrogenation, and column chromatography after beta-elimination, show that the polysaccharide is covalently bound to the protein.

描述了一种基于粘多糖 - 十六烷基吡啶络合物的溶解性，从牛肝包膜中分离和纯化肝素 - 蛋白质复合物的方法。2. 用这种方法得到的肝素 - 蛋白质复合物的产量平均为每100克干牛肝包膜35毫克。3. 多糖的分析结果与先前发表的纯化肝素质级分的值吻合良好。4. 蛋白质成分的氨基酸分析显示出与软骨粘蛋白的一些相似之处。5. β-羰基消除反应（无论有无催化氢化）以及消除反应后的柱色谱分析结果表明，多糖与蛋白质是共价结合的。

Serafini-Fracassini A, Durward J J, Floreani L

Biochem J. 1969 Apr;112(2):167-72. doi: 10.1042/bj1120167.

A procedure for the isolation and purification of the heparin-protein complex from ox liver capsule, based on the solubility properties of mucopolysaccharide-cetylpyridinium complexes, is described. 2. The yield of the heparin-protein complex with this method averages 35mg./100g. of dry ox liver capsule. 3. The results of analyses on the polysaccharide show good agreement with values previously published for purified heparin fractions. 4. The amino acid analysis of the protein component shows several similarities to that of chondromucoprotein. 5. The results of beta-carbonyl elimination, either with or without catalytic hydrogenation, and column chromatography after beta-elimination, show that the polysaccharide is covalently bound to the protein.

描述了一种基于粘多糖 - 十六烷基吡啶络合物的溶解性，从牛肝包膜中分离和纯化肝素 - 蛋白质复合物的方法。2. 用这种方法得到的肝素 - 蛋白质复合物的产量平均为每100克干牛肝包膜35毫克。3. 多糖的分析结果与先前发表的纯化肝素质级分的值吻合良好。4. 蛋白质成分的氨基酸分析显示出与软骨粘蛋白的一些相似之处。5. β-羰基消除反应（无论有无催化氢化）以及消除反应后的柱色谱分析结果表明，多糖与蛋白质是共价结合的。