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游离多核糖体制剂中的无颗粒膜及其对蛋白质生物合成研究可能产生的干扰。

Agranular membranes in free polysome preparations and their possible interference in studies of protein biosynthesis.

作者信息

Murty C N, Hallinan T

出版信息

Biochem J. 1969 Apr;112(3):269-74. doi: 10.1042/bj1120269.

DOI:10.1042/bj1120269
PMID:5801301
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1187705/
Abstract
  1. Phospholipid-rich membranous contaminants are present in free polysomes from rat liver isolated on discontinuous sucrose gradients. 2. Electron-microscopic studies indicate that the membranous contaminants are mainly agranular with very occasional granular membranes. This is confirmed by the study of their sedimentation behaviour and their initial rate of labelling with radioactive glucosamine in vivo. 3. Conventional ribosome-purification methods fail to remove the contaminants, whereas deoxycholate effectively solubilizes the membranous contaminants with little breakdown of polysomes. 4. Amino acid-incorporation studies show that these membranous contaminants may seriously interfere in assessment of the functional capacity of free polysomes in protein biosynthesis in vivo.
摘要
  1. 在通过不连续蔗糖梯度分离得到的大鼠肝脏游离多核糖体中存在富含磷脂的膜性污染物。2. 电子显微镜研究表明，膜性污染物主要是无颗粒的，偶尔有颗粒状膜。对其沉降行为及其在体内用放射性葡糖胺标记的初始速率的研究证实了这一点。3. 传统的核糖体纯化方法无法去除污染物，而脱氧胆酸盐能有效溶解膜性污染物，且多核糖体几乎不被破坏。4. 氨基酸掺入研究表明，这些膜性污染物可能会严重干扰对游离多核糖体在体内蛋白质生物合成中功能能力的评估。
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48a6/1187705/1317345548cf/biochemj00704-0018-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48a6/1187705/aad55518abfb/biochemj00704-0017-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48a6/1187705/1317345548cf/biochemj00704-0018-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48a6/1187705/aad55518abfb/biochemj00704-0017-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48a6/1187705/1317345548cf/biochemj00704-0018-a.jpg

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Agranular membranes in free polysome preparations and their possible interference in studies of protein biosynthesis.游离多核糖体制剂中的无颗粒膜及其对蛋白质生物合成研究可能产生的干扰。
Biochem J. 1969 Apr;112(3):269-74. doi: 10.1042/bj1120269.
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引用本文的文献

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Protein synthesis by membrane-bound and free ribosomes of secretory and non-secretory tissues.分泌性和非分泌性组织的膜结合核糖体和游离核糖体的蛋白质合成。
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An improved cell fractionation procedure for the preparation of rat liver membrane-bound ribosomes.一种用于制备大鼠肝脏膜结合核糖体的改进细胞分级分离程序。
J Cell Biol. 1973 Jan;56(1):191-205. doi: 10.1083/jcb.56.1.191.
3
Localization of polysome-bound albumin and serine dehydratase in rat liver cell fractions.大鼠肝细胞组分中多核糖体结合白蛋白和丝氨酸脱水酶的定位

本文引用的文献

1
The estimation of glycogen with the anthrone reagent.用蒽酮试剂对糖原进行测定。
Arch Biochem. 1950 Jan;25(1):191-200.
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
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BIOSYNTHESIS OF GLYCOPROTEINS. IV. THE SUBCELLULAR SITES OF INCORPORATION OF GLUCOSAMINE-1-14-C INTO GLYCOPROTEIN RAT LIVER.糖蛋白的生物合成。IV. 葡糖胺-1-¹⁴C掺入大鼠肝脏糖蛋白的亚细胞定位
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Membrane-bound ribosomes of myeloma cells. I. Preparation of free and membrane-bound ribosomal fractions. Assessment of the methods and properties of the ribosomes.骨髓瘤细胞膜结合核糖体。I. 游离及膜结合核糖体组分的制备。核糖体方法及性质评估。
J Cell Biol. 1975 Oct;67(1):1-15. doi: 10.1083/jcb.67.1.1.
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Initiation factors in protein synthesis by free and membrane-bound polyribosomes of liver and hepatoma.肝脏和肝癌细胞中游离及膜结合多核糖体进行蛋白质合成的起始因子。
Biochem J. 1975 Oct;152(1):143-51. doi: 10.1042/bj1520143.
J Biol Chem. 1965 May;240:1882-8.
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POLYRIBOSOMES IN RAT TISSUES. I. A STUDY OF IN VIVO PATTERNS IN LIVER AND HEPATOMAS.大鼠组织中的多核糖体。I. 肝脏和肝癌的体内模式研究。
Cancer Res. 1964 Aug;24:1229-37.
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PREPARATION OF RAT-LIVER POLYSOMES WITHOUT THE UTILIZATION OF DETERGENT.不使用去污剂制备大鼠肝脏多核糖体
Biochim Biophys Acta. 1964 May 18;87:177-80. doi: 10.1016/0926-6550(64)90064-7.
6
Ribosomal aggregate engaged in protein synthesis: characterization of the ergosome.参与蛋白质合成的核糖体聚集体：多核蛋白体的表征
Nature. 1963 Feb 2;197:430-5. doi: 10.1038/197430a0.
7
The isolation of microsomal membranes.微粒体膜的分离
Biochem Soc Symp. 1963;22:4-31.
8
Ribonucleoprotein from a rat tumor, the Jensen sarcoma. III. Ribosomes purified without deoxycholate but with bentonite as ribonuclease inhibitor.来自大鼠肿瘤（詹森肉瘤）的核糖核蛋白。III. 未用脱氧胆酸盐但用膨润土作为核糖核酸酶抑制剂纯化的核糖体。
J Biol Chem. 1963 Jan;238:318-23.
9
Glycogen synthesis from uridine diphosphate glucose. The distribution of the enzyme in liver cell fractions.由尿苷二磷酸葡萄糖合成糖原。该酶在肝细胞各组分中的分布。
J Biophys Biochem Cytol. 1961 Jun;10(2):195-209. doi: 10.1083/jcb.10.2.195.
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Destruction of reticulocyte ribosomes by deoxycholate.
Biochim Biophys Acta. 1967 Sep 26;145(2):506-8. doi: 10.1016/0005-2787(67)90069-x.