一种用于研究体内正常钙化起始的模型。
A model for studying the initiation of normal calcification in vivo.
作者信息
Alcock N W, Rd J A
出版信息
Biochem J. 1969 May;112(4):511-3. doi: 10.1042/bj1120511.
Abstract
- Rat costal cartilage was found to begin to calcify normally when the rats weigh 35-45g. 2. The cartilage is suggested as a model for the study in vivo of mechanisms concerned with normal calcification. 3. The model was tested by studying the incorporation of fluoride into the mineral deposited in the tissue. 4. The percentage of inorganic material in cartilage rose from approx. 3% of the dry weight in the uncalcified tissue to 62% in the tissue from rats weighing 300g. 5. Mineral deposited had a calcium/phosphorus molar ratio of 1.65. 6. After the oral administration of sodium fluoride to rats, fluoride was incorporated into cartilage mineral. 7. The concentration of fluoride in cartilage ash increased rapidly with calcification and the mineral became more highly fluoridated than the corresponding rib bone. 8. Fluoridated mineral showed a marked decrease in citrate concentration.
摘要
- 发现当大鼠体重为35 - 45克时，其肋软骨开始正常钙化。2. 该软骨被建议作为体内研究正常钙化相关机制的模型。3. 通过研究氟化物掺入组织中沉积的矿物质来测试该模型。4. 软骨中无机物质的百分比从未钙化组织干重的约3%上升到体重300克大鼠组织中的62%。5. 沉积的矿物质钙/磷摩尔比为1.65。6. 给大鼠口服氟化钠后，氟化物掺入软骨矿物质中。7. 随着钙化，软骨灰分中的氟化物浓度迅速增加，且该矿物质的氟化程度高于相应的肋骨。8. 氟化矿物质中的柠檬酸盐浓度显著降低。
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