测定食品中维生素B6的简化荧光法。

Simplified fluorimetric methods for determination of vitamin B6 in food materials.

作者信息

Fiedlerová V, Davídek J

出版信息

Z Lebensm Unters Forsch. 1978 Feb 24;166(2):93-5. doi: 10.1007/BF01267784.

Abstract

A simple fluorimetric method for the determination of vitamin B6 in food materials is proposed, enabling total vitamin B6 to be determined after the chromatographic removal of interfering substances on Dowex 50 WX 8 columns. Pyridoxol, pyridoxal and pyridoxamine are transferred into the highly fluorescent lactone of 4-pyridoxic acid. The method is specific and agrees well with the microbilogical assay. Three or four determinations can be performed in eight hours.

摘要

提出了一种测定食品材料中维生素B6的简单荧光法，该方法能在Dowex 50 WX 8柱上通过色谱法去除干扰物质后测定总维生素B6。吡哆醇、吡哆醛和吡哆胺被转化为4-吡哆酸的高荧光内酯。该方法具有特异性，与微生物测定法结果吻合良好。八小时内可进行三到四次测定。