Bostian K A, Blackburn B S, Wannemacher R W, McGann V G, Beisel W R, Dupont H L
J Lab Clin Med. 1976 Apr;87(4):577-85.
An automated immunoprecipitin system has been utilized to quantitate the concentration of 10 specific proteins in the plasma of man. Values obtained by this technique are in agreement with the published concentrations for these specific plasma proteins. This technique was utilized to determine the sequential change s in 10 individual plasma proteins of volunteers exposed to Salmonella typhi. In those volunteers who developed typical typhoid fever, plasma concentrations of the acute phase proteins, alpha1-acid glycoprotein, alpha1-antitrypsin, and haptoglobin, as well as C3 complement were significantly increased with the onset of febrile illness. In contrast, the concentration of plasma albumin and tranferrin were depressed while plasma IgM became elevated during early convalescence from this infection. No significant changes were observed in the plasma concentrations of alpha2-macroglobulin, IgG, or IgA. In the exposed volunteers who did not become ill, the only significant change was a brief depression of alpha1-antitrypsin. During typhoid fever the patterns of change for individual plasma acute-phase globulins were different from those reported for patients with hepatitis, myocaridal infarction, or surgery.
一种自动免疫沉淀系统已被用于定量人血浆中10种特定蛋白质的浓度。通过该技术获得的值与这些特定血浆蛋白已公布的浓度一致。该技术被用于确定暴露于伤寒沙门氏菌的志愿者的10种个体血浆蛋白的顺序变化。在那些患典型伤寒热的志愿者中，急性期蛋白、α1-酸性糖蛋白、α1-抗胰蛋白酶和触珠蛋白以及C3补体的血浆浓度随着发热疾病的发作而显著增加。相比之下，血浆白蛋白和转铁蛋白的浓度降低，而在从这种感染的早期恢复期血浆IgM升高。在α2-巨球蛋白、IgG或IgA的血浆浓度中未观察到显著变化。在未患病的暴露志愿者中，唯一显著的变化是α1-抗胰蛋白酶的短暂降低。在伤寒热期间，个体血浆急性期球蛋白的变化模式与肝炎、心肌梗死或手术患者报道的不同。
