Faras A J, Erikson R L
J Virol. 1969 Jul;4(1):31-5. doi: 10.1128/JVI.4.1.31-35.1969.
"L-cell virion," obtained from chronically infected 929 L-cell lines, was purified on 70% sucrose pads and its ribonucleic acid (RNA) was extracted and analyzed on sucrose gradients. Two RNA components were isolated from the virion, a rapidly sedimenting species (82S) and a more slowly sedimenting species (6S). When the fast-moving component was heated to 45 C, it yielded a homogeneous peak which now sedimented at approximately 72S. Upon heating to 70 C, the peak dissociates into a smaller homogeneous RNA species with a sedimentation coefficient of approximately 33S. Ribonuclease treatment and base composition analysis revealed that the fast-moving species is a single-stranded molecule. Base ratio analysis also revealed that the small (6S) RNA component is similar in its base composition to L-cell ribosomal RNA and distinctly different from the base composition of the large (72S) RNA component. Actinomycin D inhibits production of the L-cell virion by the L-cell line.
从长期感染的929 L细胞系中获得的“L细胞病毒粒子”，在70%蔗糖垫层上进行纯化，提取其核糖核酸（RNA）并在蔗糖梯度上进行分析。从病毒粒子中分离出两种RNA成分，一种沉降速度快的（82S）和一种沉降速度慢的（6S）。当快速移动的成分加热到45℃时，它产生一个均匀的峰，此时沉降系数约为72S。加热到70℃时，该峰解离成沉降系数约为33S的较小均匀RNA种类。核糖核酸酶处理和碱基组成分析表明，快速移动的种类是单链分子。碱基比率分析还表明，小的（6S）RNA成分的碱基组成与L细胞核糖体RNA相似，与大的（72S）RNA成分的碱基组成明显不同。放线菌素D抑制L细胞系产生L细胞病毒粒子。
