Lake B D, Puri P, Nixon H H, Claireaux A E
Arch Pathol Lab Med. 1978 May;102(5):244-7.
Suction rectal biopsy specimens, from a series of 168 infants and children with constipation and other gastrointestinal problems, were stained with a sensitive acetylcholinesterase method, and the results were compared with routine histology, radiology, anorectal manometry, and the final diagnosis. In all cases of Hirschsprung's disease, there was an increase in numbers and sizes of cholinergic nerves in the lamina propria and muscularis mucosae. No false-positive or false-negative results were found. The test was found to be more reliable and consistent than other methods available.
对168例患有便秘及其他胃肠道问题的婴幼儿和儿童的直肠活检抽吸标本，采用一种灵敏的乙酰胆碱酯酶方法进行染色，并将结果与常规组织学、放射学、肛门直肠测压法以及最终诊断结果进行比较。在所有先天性巨结肠病例中，固有层和黏膜肌层中胆碱能神经的数量和大小均增加。未发现假阳性或假阴性结果。该检测方法比其他现有方法更可靠、更一致。
