Bilezikian S B, Laleli Y, Tsan M F, Hodkinson B A, Ice S, McIntyre P A
Johns Hopkins Med J. 1976 Apr;138(4):124-9.
A method has previously been described which detected xenogeneic and allogeneic antibodies to human granulocytes by their inhibition of the normal phagocytosis-associated hexose monophosphate shunt (HMS) activity. This method was used to study three patients with acute agranulocytosis secondary to antithyroid drug administration. Two patients with methimazole and one patient with propylthiouracil induced agranulocytosis were studied. Serum samples from each of these three patients taken during the acute phase of agranulocytosis had inhibitory effects on phagocytosis-associated HMS activity in leukocytes from both normal donors and the patients after their full recovery from agranulocytosis. IgM but not IgG prepared from acute sera in two patients was also inhibitory. Disruption of IgM disulfide bonds by dithiothreitol destroyed its inhibitory activity. The possibility of drug-dependent immune destruction of leukocytes in these patients is discussed.
此前已描述了一种方法，该方法通过抑制正常的吞噬相关己糖磷酸分流（HMS）活性来检测针对人粒细胞的异种和同种异体抗体。该方法用于研究3例抗甲状腺药物所致急性粒细胞缺乏症患者。研究了2例因甲巯咪唑和1例因丙硫氧嘧啶诱发粒细胞缺乏症的患者。这3例患者在粒细胞缺乏症急性期采集的血清样本，对正常供者以及粒细胞缺乏症完全康复后的患者白细胞的吞噬相关HMS活性均有抑制作用。2例患者急性期血清制备的IgM具有抑制作用，而IgG则无。二硫苏糖醇破坏IgM二硫键后，其抑制活性消失。文中讨论了这些患者白细胞药物依赖性免疫破坏的可能性。
