牛乳脂肪球膜中碱不稳定寡糖的分离与结构表征
Isolation and structural characterization of alkali-labile oligosaccharides from bovine milk-fat-globule membrane.
作者信息
Farrar G H, Harrison R
出版信息
Biochem J. 1978 Jun 1;171(3):549-57. doi: 10.1042/bj1710549.
Treatment of sialoglycopeptides derived from bovine milk-fat-globule membrane with alkaline borohydride released a reduced oligosaccharide fraction from which a tetrasaccharide and two trisaccharides were isolated. Periodate-oxidation studies coupled with methylation analysis and g.l.c.-mass spectrometry established their structures as: N-acetylneuraminyl-(2 leads to 3)-beta-D-galactopyranosyl-(1 leads to 3)-[N-acetylneuraminyl-(2 leads to 6)]-N-acetyl-D-galactosaminitol, N-acetylneuraminyl-(2 leads to 3)-D-glactopyranosyl-(1 leads to 3)-N-acetyl-D-galactosaminitol and beta-D-galactopyranosyl-(1 leads to 3)-[N-acteylneuraminyl-(2 leads to 6)]-N-acetyl-D-galactosaminitol respectively.
用碱性硼氢化物处理源自牛乳脂肪球膜的唾液酸糖肽，释放出一种还原寡糖组分，从中分离出一种四糖和两种三糖。高碘酸盐氧化研究结合甲基化分析和气相色谱 - 质谱联用确定了它们的结构分别为：N - 乙酰神经氨酸基 - (2→3) - β - D - 吡喃半乳糖基 - (1→3) - [N - 乙酰神经氨酸基 - (2→6)] - N - 乙酰 - D - 半乳糖胺醇、N - 乙酰神经氨酸基 - (2→3) - D - 吡喃葡萄糖基 - (1→3) - N - 乙酰 - D - 半乳糖胺醇和β - D - 吡喃半乳糖基 - (1→3) - [N - 乙酰神经氨酸基 - (2→6)] - N - 乙酰 - D - 半乳糖胺醇。
Zotero 插件