Thomas D B, Winzler R J
Biochem J. 1971 Aug;124(1):55-9. doi: 10.1042/bj1240055.
Studies have been made on the oligosaccharide residues of the alkali-stable carbohydrate-protein linkage of sialoglycopeptides derived from human erythrocytes. Four glycopeptides were isolated after alkaline borohydride treatment and Pronase digestion of MN-active sialoglycopeptides. The structure of one of these glycopeptides (GPIV) has been studied by sequential hydrolysis with specific glycosidases. Glycopeptide GPIV contained (per mol): 1mol of fucose, 1mol of sialic acid, 3mol of galactose, 3mol of mannose, 4mol of acetylglucosamine, 1mol of aspartic acid and fractional amounts of threonine, serine and glycine. The molecular weight of the glycopeptide was estimated to be 2330 by gel filtration. On the basis of glycosidase-digestion results, a tentative structure is proposed for the oligosaccharide moiety of glycopeptide GPIV.
人们对源自人红细胞的唾液酸糖肽的碱稳定碳水化合物-蛋白质连接的寡糖残基进行了研究。对MN活性唾液酸糖肽进行碱性硼氢化物处理和链霉蛋白酶消化后,分离出了四种糖肽。其中一种糖肽(GPIV)的结构已通过用特定糖苷酶进行顺序水解来研究。糖肽GPIV每摩尔含有:1摩尔岩藻糖、1摩尔唾液酸、3摩尔半乳糖、3摩尔甘露糖、4摩尔乙酰葡糖胺、1摩尔天冬氨酸以及少量的苏氨酸、丝氨酸和甘氨酸。通过凝胶过滤法估计该糖肽的分子量为2330。根据糖苷酶消化结果,提出了糖肽GPIV寡糖部分的初步结构。