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Arch Biochem Biophys. 1967 Mar;119(1):411-9. doi: 10.1016/0003-9861(67)90472-9.

4

Immune reactions in polysaccharide media. Investigation on complex-formation between some polysaccharides, albumin and immunoglobulin G.

Biochem J. 1969 May;112(4):483-7. doi: 10.1042/bj1120483.

5

Immune reactions in polysaccharide media. Experiments with specific antibodies of different affinities for serum albumin.

Biochem J. 1969 Aug;114(1):151-5. doi: 10.1042/bj1140151.

6

Immune reactions in polysaccharide media. Polysaccharide-enhanced precipitin reactions with antigens of various sizes.

Biochem J. 1969 Aug;114(1):145-9. doi: 10.1042/bj1140145.

7

Immune reactions in polysaccharide media. The effect of hyaluronate, chondroitin sulphate and chondroitin sulphate-protein complex on the precipitin reaction.

Biochem J. 1969 May;112(4):475-81. doi: 10.1042/bj1120475.

8

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Acta Chem Scand. 1966;20(5):1251-62. doi: 10.3891/acta.chem.scand.20-1251.

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多糖介质中的免疫反应。沉淀素反应中抗原-抗体复合物的组成。

Immune reactions in polysaccharide media. The composition of the antigen-antibody complexes in the precipitin reaction.

作者信息

Hellsing K

出版信息

Biochem J. 1969 Aug;114(1):141-4. doi: 10.1042/bj1140141.

DOI:10.1042/bj1140141

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1184806/

Abstract

The precipitin reaction is enhanced in the presence of polysaccharides (Hellsing, 1966). This reaction has now been studied in detail with labelled antigen ((125)I-labelled human serum albumin) and antibody ((131)I-labelled rabbit anti-albumin immunoglobulin G). The relative proportions of antigen and antibody in the precipitates are unchanged by the addition of dextran in spite of the increased precipitation. The ratio of antibody to antigen in the soluble immune complexes decreases with increasing polysaccharide concentration. This can be interpreted as a decrease in the aggregate size of the complexes. At the same time the amount of free antigen in the solution increases. The results are consistent with a decrease in solubility, primarily of the large immune aggregates, together with a shift in the equilibrium between small and large complexes. The effect is in accord with a steric-exclusion phenomenon.

摘要

在多糖存在的情况下，沉淀素反应会增强（赫尔辛，1966年）。现在已使用标记抗原（（125）I标记的人血清白蛋白）和抗体（（131）I标记的兔抗白蛋白免疫球蛋白G）对该反应进行了详细研究。尽管沉淀增加，但沉淀物中抗原和抗体的相对比例不会因添加葡聚糖而改变。随着多糖浓度的增加，可溶性免疫复合物中抗体与抗原的比例会降低。这可以解释为复合物聚集体大小的减小。与此同时，溶液中游离抗原的量会增加。结果与溶解度降低一致，主要是大免疫聚集体的溶解度降低，同时小复合物和大复合物之间的平衡发生了转移。这种效应符合空间排阻现象。