多糖介质中的免疫反应。使用对血清白蛋白具有不同亲和力的特异性抗体进行的实验。
Immune reactions in polysaccharide media. Experiments with specific antibodies of different affinities for serum albumin.
作者信息
Hellsing K
出版信息
Biochem J. 1969 Aug;114(1):151-5. doi: 10.1042/bj1140151.
Abstract
Rabbit antibody fractions of different affinities for human serum albumin were prepared by an immunosorbent technique. The fractions were used in studies on the enhancement of the precipitin reaction by polymers. Dextran increased the immune precipitation to about the same extent regardless of whether antibodies with high and low affinities were used. The effect should considerably facilitate the detection of antibodies with low precipitating ability in immunological assays. The results are discussed in terms of a steric-exclusion mechanism.
摘要
通过免疫吸附技术制备了对人血清白蛋白具有不同亲和力的兔抗体组分。这些组分用于研究聚合物对沉淀素反应的增强作用。无论使用高亲和力还是低亲和力的抗体，葡聚糖都能使免疫沉淀增加到大致相同的程度。这一效应应能极大地促进在免疫测定中检测沉淀能力低的抗体。从空间排阻机制的角度对结果进行了讨论。
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