胆固醇生物合成中δ8-胆甾烯醇-δ7-胆甾烯醇异构酶反应的可逆性
The reversibility of the delta8-cholestenol-delta7-cholestenol isomerase reaction in cholesterol biosynthesis.
作者信息
Wilton D C, Rahimtula A D, Akhtar M
出版信息
Biochem J. 1969 Aug;114(1):71-3. doi: 10.1042/bj1140071.
Abstract
The incubation of Delta(7)-cholestenol with a 10000g supernatant or 105000g microsomes in the presence of tritiated water is studied. The reisolated Delta(7)-cholestenol contained up to 0.67g.atom of tritium/mole. This result can best be explained by assuming the reversibility of the reaction Delta(8)-cholestenol right harpoon over left harpoon Delta(7)-cholestenol.
摘要
研究了在氚化水存在的情况下，将δ(7)-胆甾烯醇与10000g上清液或105000g微粒体一起温育的情况。重新分离得到的δ(7)-胆甾烯醇每摩尔含有高达0.67克原子的氚。通过假设反应δ(8)-胆甾烯醇⇌δ(7)-胆甾烯醇的可逆性，可以最好地解释这一结果。
相似文献
1
The reversibility of the delta8-cholestenol-delta7-cholestenol isomerase reaction in cholesterol biosynthesis.
Biochem J. 1969 Aug;114(1):71-3. doi: 10.1042/bj1140071.
2
Enzymatic conversion of 32-oxygenated delta-7-lanosterol derivatives and of delta-8(14)-4,4-dimethyl-cholestenol to cholesterol.
Biochem Biophys Res Commun. 1968 Aug 13;32(3):568-74. doi: 10.1016/0006-291x(68)90701-8.
3
The role of a cholesta-8,14-dien-3-beta-ol system in cholesterol biosynthesis.
Biochem J. 1969 Mar;111(5):757-61. doi: 10.1042/bj1110757.
4
The formation and reduction of the 14,15-double bond in cholesterol biosynthesis.
Biochem J. 1971 Jan;121(1):131-7. doi: 10.1042/bj1210131.
5
4,4-dimethyl-5-alpha-cholesta-8,14-dien-3-beta-ol. A new precursor of cholesterol in mammalian tissues.
Life Sci. 1969 Jun 15;8(12):629-34. doi: 10.1016/0024-3205(69)90219-7.
6
The stereochemistry of the hydrogen elimination in the biological conversion of cholest-7-en-3-beta-ol into cholesterol.
Biochem J. 1967 Feb;102(2):462-7. doi: 10.1042/bj1020462.
7
5 Alpha-cholest-8(14)-en-3 beta-ol, a possible intermediate in the biosynthesis of cholesterol. Enzymatic conversion to cholesterol and isolation from rat skin.
J Biol Chem. 1969 Oct 25;244(20):5440-8.
8
The intracellular organisation of cholesterol biosynthesis. A review.
Steroids Lipids Res. 1972;3(4):236-56.
9
The conversion of steroid 7,9-dienes into cholesterol.
Biochem J. 1969 May;112(4):545-6. doi: 10.1042/bj1120545.
10
C29-carboxylic acid intermediates of cholesterol biosynthesis in rat liver.
Biochem J. 1971 Mar;122(1):24P-25P. doi: 10.1042/bj1220024pc.
引用本文的文献
1
Structural basis for human sterol isomerase in cholesterol biosynthesis and multidrug recognition.
Nat Commun. 2019 Jun 5;10(1):2452. doi: 10.1038/s41467-019-10279-w.
2
Design of high energy intermediate analogues to study sterol biosynthesis in higher plants.
Lipids. 1986 Jan;21(1):52-62. doi: 10.1007/BF02534303.
3
TM6SF2 and MAC30, new enzyme homologs in sterol metabolism and common metabolic disease.
Front Genet. 2014 Dec 11;5:439. doi: 10.3389/fgene.2014.00439. eCollection 2014.
4
Characterization of plant carotenoid cyclases as members of the flavoprotein family functioning with no net redox change.
Plant Physiol. 2010 Jul;153(3):970-9. doi: 10.1104/pp.110.155440. Epub 2010 May 11.
5
Crystal structure of isopentenyl diphosphate:dimethylallyl diphosphate isomerase.
EMBO J. 2001 Apr 2;20(7):1530-7. doi: 10.1093/emboj/20.7.1530.
6
Silver ion high pressure liquid chromatography provides unprecedented separation of sterols: application to the enzymatic formation of cholesta-5,8-dien-3 beta-ol.
Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11603-8. doi: 10.1073/pnas.93.21.11603.
7
Different approaches toward an automatic structural alignment of drug molecules: applications to sterol mimics, thrombin and thermolysin inhibitors.
J Comput Aided Mol Des. 1994 Dec;8(6):751-78. doi: 10.1007/BF00124019.
8
The stereochemistry of hydrogen elimination during 7,8-double bond formation by Tetrahymena pyriformis.
Biochem J. 1970 Feb;116(3):337-9. doi: 10.1042/bj1160337.
9
The stereochemistry of hydrogen elimination from C-7 in cholesterol and ergosterol biosynthesis.
Biochem J. 1970 Apr;117(3):539-42. doi: 10.1042/bj1170539.
本文引用的文献
1
The biological conversion of 7-dehydrocholesterol into cholesterol and comments on the reduction of double bonds.
Biochem J. 1968 Feb;106(4):803-10. doi: 10.1042/bj1060803.
Zotero 插件