胆固醇生物合成中δ8-胆甾烯醇-δ7-胆甾烯醇异构酶反应的可逆性
The reversibility of the delta8-cholestenol-delta7-cholestenol isomerase reaction in cholesterol biosynthesis.
作者信息
Wilton D C, Rahimtula A D, Akhtar M
出版信息
Biochem J. 1969 Aug;114(1):71-3. doi: 10.1042/bj1140071.
Abstract
The incubation of Delta(7)-cholestenol with a 10000g supernatant or 105000g microsomes in the presence of tritiated water is studied. The reisolated Delta(7)-cholestenol contained up to 0.67g.atom of tritium/mole. This result can best be explained by assuming the reversibility of the reaction Delta(8)-cholestenol right harpoon over left harpoon Delta(7)-cholestenol.
摘要
研究了在氚化水存在的情况下，将δ(7)-胆甾烯醇与10000g上清液或105000g微粒体一起温育的情况。重新分离得到的δ(7)-胆甾烯醇每摩尔含有高达0.67克原子的氚。通过假设反应δ(8)-胆甾烯醇⇌δ(7)-胆甾烯醇的可逆性，可以最好地解释这一结果。
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