银离子高效液相色谱法实现了甾醇前所未有的分离：应用于胆甾-5,8-二烯-3β-醇的酶促合成。
Silver ion high pressure liquid chromatography provides unprecedented separation of sterols: application to the enzymatic formation of cholesta-5,8-dien-3 beta-ol.
作者信息
Ruan B, Shey J, Gerst N, Wilson W K, Schroepfer G J
机构信息
Department of Chemistry, Rice University, Houston, TX 77251-1892, USA.
出版信息
Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11603-8. doi: 10.1073/pnas.93.21.11603.
Abstract
We report that silver ion HPLC provides remarkable separations of C27 sterols differing only in the number or location of olefinic double bonds. This technique has been extended to LC-MS, analysis of purified components by GC, GC-MS, and 1H NMR, and to its use on a semipreparative scale. The application of this methodology for the demonstration of the catalysis, by rat liver microsomes, of the conversion of 7-dehydrocholesterol to cholesta-5,8-dien-3 beta-ol is also presented.
摘要
我们报告银离子高效液相色谱法能对仅在烯键式双键数量或位置上存在差异的C27甾醇实现出色的分离。该技术已扩展至液相色谱 - 质谱联用、通过气相色谱、气相色谱 - 质谱联用和氢核磁共振对纯化成分进行分析，以及用于半制备规模。本文还介绍了该方法在证明大鼠肝脏微粒体催化7 - 脱氢胆固醇转化为胆甾 - 5,8 - 二烯 - 3β - 醇方面的应用。
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